Neutral buffered formalin 5 - 10 min.  Rinse off culture media containing
serum with pure culture medium, or you fix the serum to the cells,
potential background problem.


t 04:45 PM 4/17/01 -0700, you wrote:
>Has anybody done an H&E stain on cell cultures grown in 24-well plates?
>And how do you fix them? 
>Thank you for any help...
>
>Lalen Alsing
>UC Davis - CAHFS, Fresno
>
>
>
>
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)