Bouin's fixative for 1 minute, 70% ethanol 2 x 1min, tap water, then H+E.
Works fine.

         Regards,   Laurie.



At 04:45  17/04/01 -0700, Elena Alsing wrote:
>Has anybody done an H&E stain on cell cultures grown in 24-well plates?
>And how do you fix them? 
>Thank you for any help...
>
>Lalen Alsing
>UC Davis - CAHFS, Fresno
>
>
>
Mr.Laurie Reilly 
Histotechnologist.                         Ph 07 4781 4468
Physiology & Pharmacology                 Fax  07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.    
James Cook University                     laurie.reilly@jcu.edu.au
       Townsville  Qld. 4811                            
Australia.