Elias states 0.6% wt/vol, did not give the solvent, assuming PBS, 7 - 15
min RT, followed by 7 - 15 min at 57C (ficin took 7 min).  Man this is one
nasty dude of a chemical to work with, any chance of finding something
nicer to do the job????


At 01:44 PM 4/30/01 -0600, you wrote:
>HELP - I NEED A RECIPE FOR FICIN - I WOULD LIKE TO KEEP THE SOLUTION PREMADE
>IN FRIDGE FOR APPROXIMATELY ONE MONTH - I WILL HAVE TO USE FAIRLY LARGE
>QUANTITIES - 50 ML AT A TIME - ANYONE?
>SHANNON KLASSEN
>IHC TECH
>SASKATOON DISTRICT HEALTH
>SASKATOON, CANADA
>
>
>
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)