Elsie,

Make sure you are using alcohol that has been denatured with methanol and 
isopropyl which is known as Reagent Alcohol. Stay away from  alcohols 
denatured with hydrocarbons and ethyl acetate.

In my past life, I never used anything else but reagent alcohol and there 
was never a problem.

Rande Kline
EM Science

>From: Elsie_Kok@notes.ttsh.gov.sg
>To: histonet@pathology.swmed.edu
>Subject: Denatured alcohol
>Date: Wed, 18 Apr 2001 17:23:26 +0800
>
>
>
>
>From: Elsie Kok@TTSH on 18/04/2001 05:23 PM
>
>Hi
>
>Is anybody out there use denatured alcohol to process tissue? Do you have 
>any
>problem with sectioning and staining (both H&E and IHC)?We have started to 
>use
>the denatured alcohol for a couple of weeks but have problem with 
>sectioning and
>staining.
>
>Elsie kok
>Senior Medical Technologist
>Singapore
>
>
>

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