Re: Calretinin HELP

From:Andickert@aol.com


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We pretreat all tissue for IHC with Declere. we did 6 months of various 
methods considering quality, expense and time, Declere won out in the end and 
believe me I fought against it because of using the pressure cooker, however, 
working closely with my pathologist very closely for those 6 months the 
pressure cooker is in!!!! We never have a problem with staining, that is if 
the antibody has not leaked completely out of the dispenser, if you know what 
I mean. 

I spoke with Ventana tech service today, mid June new dispensers!!!!!!!


Ann Dickert
Southern Pathology @ Parkridge Medical Center
423-493-6840


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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>We pretreat all tissue for IHC with Declere. we did 6 months of various 
<BR>methods considering quality, expense and time, Declere won out in the end and 
<BR>believe me I fought against it because of using the pressure cooker, however, 
<BR>working closely with my pathologist very closely for those 6 months the 
<BR>pressure cooker is in!!!! We never have a problem with staining, that is if 
<BR>the antibody has not leaked completely out of the dispenser, if you know what 
<BR>I mean. 
<BR>
<BR>I spoke with Ventana tech service today, mid June new dispensers!!!!!!!
<BR>
<BR>
<BR>Ann Dickert
<BR>Southern Pathology @ Parkridge Medical Center
<BR>423-493-6840
<BR></FONT></HTML>

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