Re: Calretinin HELP

From:Shirley Powell <powell_sa@Mercer.EDU>

Hi Jim,
I use Zymed's Calretinin, #08-4211 rabbit antibody.  This is a V-Line
predilute for the Ventana, but they have a concentrated version too if you
have other automation or do them manually.  I use the Biocare Decloaking
Chamber (pressure cooker) with Borg, and also use Declere following
manufacturer's directions for HIER (heat induced epitope retrieval).
Incubation for antibody on the NexEs is 32 minutes.  So far I have gotten
great results.  Give me a call if you need more info.

Shirley Powell

"Fessler, Jim" wrote:

> I purchased 3 different calretinin antibodies and haven't been able to get
> any of them to work....I have tried every way imaginable.....I have also
> tried 5 different mesotheliomas with each ab.......any suggestions?
> Thanks
> Jim Fessler
> Pennsylvania Hospital
> Celebrating our 250th year as the nations first hospital

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