Rapid Processing of Prostate Bx's
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Hello to all:
Looking for some advice on our short run (2 hour processing) of prostate
bx's. We SOMETIMES experience what our pathologists call "popcorn nuclei."
It looks like a bubble within the nuclei and stains lighter making it very
distracting to look at and make a diagnosis. These bx's are all well fixed (
overnight). We have tried increasing the processing time to 4 hours-more
time in alcohol's and xylene- and still SOMETIMES see this effect. Our
overnight run also on occasion demonstrates this. We have ruled out the
disease process as they are not all the same diagnosis. We use the same
drying time and staining time for all our surgical tissues. We also have a
hazy or cloudy effect on these bx's even though we use the same staining
program. Any ideas?? Also, does anyone know of another type of colored
tissue dye, besides India ink and bouins, to mark margins of tissue? We have
used the Davidson(?) red, green and yellow, but find it takes to long to dry
and gets all over everything. Is there something to set this like bouins
does to India ink? All help is appreciated.
Tina Cahak
Sacred Heart Hospital
Eau Claire, WI
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Hello to all:
<BR>Looking for some advice on our short run (2 hour processing) of prostate
<BR>bx's. We SOMETIMES experience what our pathologists call "popcorn nuclei."
<BR>It looks like a bubble within the nuclei and stains lighter making it very
<BR>distracting to look at and make a diagnosis. These bx's are all well fixed (
<BR>overnight). We have tried increasing the processing time to 4 hours-more
<BR>time in alcohol's and xylene- and still SOMETIMES see this effect. Our
<BR>overnight run also on occasion demonstrates this. We have ruled out the
<BR>disease process as they are not all the same diagnosis. We use the same
<BR>drying time and staining time for all our surgical tissues. We also have a
<BR>hazy or cloudy effect on these bx's even though we use the same staining
<BR>program. Any ideas?? Also, does anyone know of another type of colored
<BR>tissue dye, besides India ink and bouins, to mark margins of tissue? We have
<BR>used the Davidson(?) red, green and yellow, but find it takes to long to dry
<BR>and gets all over everything. Is there something to set this like bouins
<BR>does to India ink? All help is appreciated.
<BR>Tina Cahak
<BR>Sacred Heart Hospital
<BR>Eau Claire, WI</FONT></HTML>
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