Date sent:      	Mon, 23 Apr 2001 15:59:06 -0500
From:           	"Charles.Embrey" <Charles.Embrey@carle.com>
Subject:        	RE: lymph node revealing solution
To:             	"'LINDA GORMAN'" <lgorman1@kumc.edu>
Copies to:      	"'Histonet@pathology.swmed.edu'" <Histonet@pathology.swmed.edu>

> Linda's right.  It's the real (original) Carnoy's Fix.  I use it and it's
> great:
> Acetic Acid   10ml
> 100% ETOH     60ml
> Chloroform    30ml
Carle Clinic
> Urbana, IL
> 


I am sure it is, but you should seriously consider finding an 
alternative to using chloroform. This is a suspect carcinogen in 
man and really should not be used. I wouldn't like to see this 
fixative come back into general use, and as with mercury 
containing fixatives,should be one for the history books.
Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360