RE: hematoxylin precipitate
|From:||"Smith, Susie" <SSmith@cytologix.com>|
I would suggest that you try Mayer's Hematoxylin instead of the current one
that you use for H&E's. Precipitation of hematoxylin is a normal reaction
especially when linked to alum and filtration may solve the problem for only
a short period of time. Hope that this helps.
From: Nancy Maronto [mailto:firstname.lastname@example.org]
Sent: Thursday, April 19, 2001 5:59 PM
Subject: hematoxylin precipitate
We have been performing BrdU staining on Dog tissue with a DAB chromogen.
Our hematoxylin nuclear stain has been leaving some areas of the slide with
what looks like precipitate. We have diluted the hematoxylin to 50% and
filtered it more than once. We also have used different products, and we
still get some of this dark blue precipitate. can anyone suggest a reason?
One of the hematoxylins is the same as what we use for our regular H & E
staining and our regular slides do not have any precipitate and turn out
beautiful. Thanks for your help
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