RE: bx turn around times
|From:||"Garrison, Becky" <firstname.lastname@example.org>|
I don't do the prolonged drying BUT I do have a routine that I follow
without fail. Grids have to be perfectly clean.
Sonicate in dilute HCl for 10-20 sec
Pour off and sonicate in 100% EtOH for 10 to 20 seconds.
Pour out grids and dry on filter paper.
The sections never fall off. I have used 200 mesh, 300 mesh, thin bars,
regular bars and hexagonal bars. Dilute HCl made by adding one drop of
conc. HCl to a jar with about 30ml of dWater. This is not an exact
measurement; just eyeball.
Dry on filter paper a minimum of 15 minutes before staining. I clean a
batch of grids each day of sectionning. If I use the previous days grids
without recleaning, the sections may or may not fall off. So I clean grids
each day of use.
From: Gayle Callis [mailto:email@example.com]
Sent: Thursday, April 26, 2001 4:04 PM
To: firstname.lastname@example.org; email@example.com
Subject: Re: bx turn around times
Our EM lab makes sure the grids with sections are air dried well before
staining, overnight and up to 2 days. Ted Pella copper grids, 150 mesh,
cleaned with nitric acid etching before picking up the section.
At 09:45 AM 4/26/01 -0500, you wrote:
>>Do you have problems keeping sections on grids? I have had problems with
>>200 mesh thin bar grids sections just fall off during staining.
>John, I use 300 copper mesh grids. If I TEM stain immediately after picking
>up ultrathins, yes all sections wash off. But placing copper grids in the
>40oC oven, seems to help adhere sections. Teresa
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
404 994-4303 (FAX)
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