RE: Tough Toenails
|From:||"Su, Phy-Huynh" <firstname.lastname@example.org>|
Have you tried to coat your slides with 3-aminopropyltriethoxysilane? We
use home made coated slides with better success than commercial ones for
large sections of bone and cartilage in our lab. You can buy the solution
from Sigma, cat. # A3648.
We use two changes of APES.
Histochemical Journal 1986:18, 271-276.
My humble experience.
> -----Original Message-----
> From: StephnJill@aol.com [SMTP:StephnJill@aol.com]
> Sent: Sunday, April 22, 2001 10:30 AM
> To: email@example.com
> Subject: Tough Toenails
> Dear all:
> I work in a dermpath lab where we receive numerous toenails for
> We have no problem with processing, the trouble occurs trying to keep the
> nails on the slide for H&E or PAS stains. There is gelatin in the water
> baths. We have used pol- poly-l-lysine slides, positively charged slides,
> Elmer's glue slides and we leave these cut slides in the oven for 1/2 hour
> an hour.
> Does anyone have secrets for toenails? I would greatly appreciate any
> Thank you
> Jill Gilbert
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