RE: Tough Toenails

From:"Su, Phy-Huynh" <>

Have you tried to coat your slides with 3-aminopropyltriethoxysilane?  We
use home made coated slides with better success than commercial ones for
large sections of bone and cartilage in our lab.  You can buy the solution
from Sigma, cat. # A3648.

We use two changes of APES.

Histochemical Journal 1986:18, 271-276.

My humble experience.

> -----Original Message-----
> From: []
> Sent:	Sunday, April 22, 2001 10:30 AM
> To:
> Subject:	Tough Toenails
> Dear all: 
> I work in a dermpath lab where we receive numerous toenails for
> processing.   
> We have no problem with processing, the trouble occurs trying to keep the 
> nails on the slide for H&E or PAS stains.  There is gelatin in the water 
> baths.  We have used pol- poly-l-lysine slides, positively charged slides,
> Elmer's glue slides and we leave these cut slides in the oven for 1/2 hour
> or 
> an hour.   
> Does anyone have secrets for toenails?  I would greatly appreciate any 
> suggestions. 
> Thank you 
> Jill Gilbert

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