RE: Rat Tissue Processing

From:"Philopena, Jennifer" <>

Hi Cyrla.
I find that perfused rat brain always processes better, but it's a laborious
task so it rarely happens here in our lab.  I fix whole unperfused brain,
both rat and mouse, for 2 days, then I come in to gross them at 4mm
thickness and let the tissue fix for another 5 days, for a total of a week
of 10% formalin fixation.  I find that better fixation provides better
I also have a TP1050, and my processing shedule is quite long - 2 hours per
station: 70%, 80%, 95%x2, 100%x2 reagent alcohol, 3xPropar, 3xparaffin 57
And turning down the temp on the water bath helps a lot.
I hope this is useful.

>===== Original Message From C H <> =====
>Hello, All
>I wonder if any of you have a protocol that you wouldn't mind sharing for
>auto-processing rat CNS tissue for paraffin embedding.  I am a novice at
>working with paraffin,using a Leica TP1050 and so far I have not found the
>right combination of steps to prevent the tissue from overly expanding and
>separating from the paraffin in the water bath.
>Any assistance would be greatly appreciated.
>Get Your Private, Free E-mail from MSN Hotmail at

 This electronic message, including its attachments, is confidential and
proprietary and is solely for the intended recipient.  If you are not the
intended recipient, this message was sent to you in error and you are hereby
advised that any review, disclosure, copying, distribution or use of this
message or any of the information included in this message by you is
unauthorized and strictly prohibited.  If you have received this electronic
transmission in error, please immediately notify the sender by reply to this
message and permanently delete all copies of this message and its
attachments in your possession.  Thank you.

<< Previous Message | Next Message >>