RE: Quick pretreatment

From:Michele Foshee <mfoshee@cellmarque.com>

Jack,

I can understand your impatience. How would you like to completely
pretreat your slides in one easy step?  With a pressure cooker and
Trilogy or Declere, one can have those slides ready for the primary
antibody in @ 30 minutes! For more information, please go to the website
posted below.  Fellow histonetters.....those of you who use these
products, let Jack hear from you, too! Pressure cooker is the ONLY way
to go for HIER.  It completely standardizes protocol and is extremely
efficient no harmful fumes either because they are biodegradeable.  Good
Luck!

Michele L. Foshee
Technical Sales Consultant
Cell Marque Corp.
http://www.cellmarque.com 
1-800-665-7284


-----Original Message-----
From: Jack Golden [mailto:countofmontehisto@yahoo.com]
Sent: Friday, April 27, 2001 9:57 AM
To: 'Histonet' (E-mail)
Subject: Quick pretreatment


My lab is having some issues with our time efficiency.
 I am finding that we are having trouble running our
slides, even with our automation, because we are
having to go back and forth switching our slides
through these xylene channels during pretreatment.  We
are getting so-so results with our microwave, but the
main concern is time.  Is there a way to cut down on
this pretreatment time?  We've already killed almost
an hour just from running the xylene/alcohol.  Any
suggestions?

Jack Golden



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