RE: Mounting Media
From: | "Plummer, Timothy B." <Plummer.Timothy@mayo.edu> |
Christine,
Most antibody companies (i.e., Dako, Vector) sell a medium which contains an
antifade medium. Molecular Probes is also a good source. You can also
prepare you own-here is my favorite formulation which also contains DAPI
(Blue fluorescent nuclear counterstain), but it can be left out.
My formulation for DABCO-DAPI Antifade medium is: 25 mg/mL
1,4-diazabicyclo[2.2.2]octane (DABCO) with 0.5mg/mL,
4,6-diamidino-2-phenylindole (DAPI), in 50% glycerol/phosphate buffered
saline, pH 8.6. DABCO is dissolved in 100% glycerol then 2X PBS at pH 8.6
is added in equal volume to the glycerol. The DAPI can be added from a
higher concentration solution stock which can be stored frozen. All
reagents can be purchased from Sigma Chemical. pH is very important.
Tim Plummer
> -----Original Message-----
> From: cmcbride@tulane.edu [SMTP:cmcbride@tulane.edu]
> Sent: Tuesday, April 24, 2001 10:34 AM
> To: histonet
> Subject: Mounting Media
>
> Hi Histonet,
>
> Does anyone know of a good mounting media to use on immunoflourecent
> slides
> with anti-fade in it?
>
> Christine
>
> Christine McBride, M.S.
> Tulane University Medical Center
> Center for Gene Therapy
> SL-99
> 1430 Tulane Ave.
> New Orleans, LA 70112
> cmcbride@tulane.edu
> Phone:(504) 988-7069
> Fax:(504) 588-5326
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