RE: Mounting Media

From:"Plummer, Timothy B." <>


Most antibody companies (i.e., Dako, Vector) sell a medium which contains an
antifade medium.  Molecular Probes is also a good source.  You can also
prepare you own-here is my favorite formulation which also contains DAPI
(Blue fluorescent nuclear counterstain), but it can be left out.

My formulation for DABCO-DAPI Antifade medium is:  25 mg/mL
1,4-diazabicyclo[2.2.2]octane (DABCO) with 0.5mg/mL,
4,6-diamidino-2-phenylindole (DAPI), in 50% glycerol/phosphate buffered
saline, pH 8.6.  DABCO is dissolved in 100% glycerol then 2X PBS at pH 8.6
is added in equal volume to the glycerol.  The DAPI can be added from a
higher concentration solution stock which can be stored frozen.  All
reagents can be purchased from Sigma Chemical.  pH is very important.

Tim Plummer

> -----Original Message-----
> From: []
> Sent:	Tuesday, April 24, 2001 10:34 AM
> To:	histonet
> Subject:	Mounting Media
> Hi Histonet,
> Does anyone know of a good mounting media to use on immunoflourecent
> slides 
> with anti-fade in it?
> Christine
> Christine McBride, M.S.
> Tulane University Medical Center
> Center for Gene Therapy
> SL-99
> 1430 Tulane Ave.
> New Orleans, LA 70112
> Phone:(504) 988-7069 
> Fax:(504) 588-5326

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