1) Remove the media from the wells.
2) Air dry 10-15 minutes
3) Apply 0.5 - 1.0 mL 10 % neutral buffered formalin directly into the
wells. Fix 15 - 30 minutes at room temp.
4) discard fixative
5) rinse gently! in water (we use a shallow tray under running water and
immerse the plates one at a time gently allowing them to fill with water x
3)
6) air dry completely
7) stain as desired. use significantly less time and or less concentrated
solutions. remember your staining one cell layer thick not a 5 um section.
Hope that helps.

Donna Montague
UAMS Orthopaedic Research
Little Rock,AR

-----Original Message-----
From: Elena Alsing [mailto:lalsing@cvdls.ucdavis.edu]
Sent: Tuesday, April 17, 2001 6:46 PM
To: histonet@pathology.swmed.edu
Subject: H&E on tissue culture in wells


Has anybody done an H&E stain on cell cultures grown in 24-well plates?
And how do you fix them? 
Thank you for any help...

Lalen Alsing
UC Davis - CAHFS, Fresno