PBS vs TRIS w/fluorescet stains

From:
Carol Ann Bobrowitz <cbobrowi@mcw.edu>

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Most procedures state using PBS when doing fluorescent staining.
Can you use Tris/HCL Buffer pH 7.6?
If not, why and why should PBS be used and at what pH?
Thanks in advance.
Carol Ann Bobrowitz
Physiology & BRI Histology Core Lab
Medical College of Wisconsin
cbobrowi@mcw.edu
(414) 456-8179

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<font size=+1>Most procedures state using PBS when doing fluorescent staining.</font>
<br><font size=+1>Can you use Tris/HCL Buffer pH 7.6?</font>
<br><font size=+1>If not, why and why should PBS be used and at what pH?</font>
<br><font size=+1>Thanks in advance.</font>
<br><font size=+1>Carol Ann Bobrowitz</font>
<br><font size=+1>Physiology & BRI Histology Core Lab</font>
<br><font size=+1>Medical College of Wisconsin</font>
<br><font size=+1>cbobrowi@mcw.edu</font>
<br><font size=+1>(414) 456-8179</font></html>

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