Elena,

I have successfully stained cultures with H&E as well as PAP. Drain
excess fluid from the slides, rinse in PBS and fix for 10min in 95%
ethanol. Stain with PAP or H&E from water (no need to dewax with
xylene!!), dehydrate, clear, mount and your done.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Elena Alsing <lalsing@cvdls.ucdavis.edu> 18/April/2001 09:45am
>>>
Has anybody done an H&E stain on cell cultures grown in 24-well
plates?
And how do you fix them? 
Thank you for any help...

Lalen Alsing
UC Davis - CAHFS, Fresno





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