|From:||Shawn Griffin <SGriffin@ameripath.com>|
From: Shawn Griffin
Sent: Friday, April 27, 2001 4:06 AM
To: 'Patti Loykasek'
Subject: RE: RCC
We use RCC, clone 66.4c2 on paraffin tissue from NovoCastra. We pretreat
the slides for 20 min using the steamer method. We use everything from
Dako. The kit we are using is LSAB2HRP. The dilution is 1:100 for 30
minutes. I hope this will help you some.
From: Patti Loykasek [mailto:firstname.lastname@example.org]
Sent: Thursday, April 26, 2001 5:11 PM
Can anyone recommend a RCC clone that works on FFPE tissue? Pretreatments?
We have tried several clones and pretreatments with inconsistent results.
Thanks for the help.
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