Cutting paraffin blocks in a cryostat

From:Andrew Gray <andrew.gray@vcp.monash.edu.au>

Hello Histonetters,

A question from a newbie to the list.   I was wondering if anyone knew
if it is possible to cut paraffin blocks in a cryostat.   I have access
to a Leica CM 1850 cryostat and I wish to examine some soft tissues of
rats.   Paraffin is preferable for handling and storage reasons, but I
don't have access to a standard microtome.   The mechanics of a cryostat
seem essentially the same, but I was wondering about the practicalities
of using it in this way (e.g. the appropriateness of the blade, holding
the block in position with the adhesive, temperature etc.).

Thanks in advance,

Andrew Gray
B.Pharm, B.PharmSc(Hons)
Ph.D student
Victorian College of Pharmacy
Melbourne, Australia
andrew.gray@vcp.monash.edu.au



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