Greetings to all.
Brief and to the point: Are colloidal irons (with/without digestion
also) known for inconsistencies? If so, are there tips to learn and
remember? Basically, we digest when requested, use 12% glacial acetic/3min.,
Colloidal Fe/1hr., back to 12% glacial acetic/3min/3changes each, and finally
into 5% Potassium Ferrocyanide-Hydrocloric Acid solution for 20 min. We
changed controls from umbilical cord to combination of pos/neg skin sections.
The problem as I understand it, is the digested slides are still staining to
much blue and with other elements staining also. I rinse the with and
without separately and thoroughly before joining them together for the
remainder of the procedure. The procedure we have on hand is from old AFIP
manual. More current literature suggest a 2% Pot/Ferr-Hcl Acid solution.
Would a 5% solution be what is making the with digestion stain more than it
should? Thoughts and comments are definetely appreciated.
Central Histology Facility
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