Bielschowsky stain

Hello everyone,

    I am seeking helpful hints in performing the Bielschowsky stain in 
reference to one particular problem I can't seem to figure out.  Why would 
any one slide in a batch of up to 4 or 5 slides not turn a golden brown?  
There are times when all my slides develop, but too often I get "hung up" on 
why some slides do not turn golden.  Indeed, the plaque and tangles may 
develop, but there are doctor's who still want the golden brown background.  
Any help is appreciated.

Deb King
Central Histology Facility
Sacramento, CA

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