methyl green is methyl blue
From: | kwalters <kwalters@blue.weeg.uiowa.edu> |
Everytime I make methyl green it turns a gorgeous shade of blue. I have a
brand new powder that I am using.
Here is my protocol: please tell me where I went wrong!!
Thanks for any help!!
Methyl Green
a ) 0.1 N Acetic Acid: Add 6 ml of glacial acetic acid to 1000 ml of
filtered distilled water.
b) 0.1 N sodium Acetate: Add 4.102 g of sodium acetate to 500 ml of distilled
water.
c) Buffer:
1)Add 755 ml of 0.1 N acetic acid to 265 ml of 0.1 N sodium
acetate.
2) Adjust pH to 4.2 with NAOH.
d) Methyl Green
1) Add 20.0 g of methyl green to the buffer solution.
2) Filter the methyl green solution.
STORAGE: room temperature
SHELF LIFE: Indefinite while in storage; when in use, the shelf life
is
2 weeks or until staining becomes too weak.
Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335-8142 / / \ \
Fax #: (319) 384-4469 ______ ((0))
email: Katherine-Walters@uiowa.edu |__| / /
|| / /
--------------
------------------
<< Previous Message | Next Message >>