methyl green is methyl blue

From:kwalters <kwalters@blue.weeg.uiowa.edu>

Everytime I make methyl green it turns a gorgeous shade of blue.  I have a 
brand new powder that I am using.

Here is my protocol:  please tell me where I went wrong!!

Thanks for any help!!

Methyl Green
a ) 0.1 N Acetic Acid: Add 6 ml of glacial acetic acid to 1000 ml of
         filtered distilled water.

b) 0.1 N sodium Acetate: Add 4.102 g of sodium acetate to 500 ml of distilled 
water.

         c) Buffer:
1)Add 755 ml of 0.1 N acetic acid to 265 ml of 0.1 N sodium 
acetate.
2) Adjust pH to 4.2 with NAOH.

         d) Methyl Green 
1) Add 20.0 g of methyl green to the buffer solution.
         		2) Filter the methyl green solution.

         STORAGE: room temperature
         SHELF LIFE: Indefinite while in storage; when in use, the shelf life 
is
         2 weeks or until staining becomes too weak.

Kathy Walters                                // 
Central Microscopy Research Facility        / /
85 EMRB                                    / /\
University of Iowa                        / /\ \
Iowa City, Iowa 52242                    / /  \ \
Phone #:  (319) 335-8142                / /    \ \
Fax #:  (319) 384-4469                ______   ((0))
email: Katherine-Walters@uiowa.edu     |__|    / /
                                        ||    / /
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