in situ on methacarn vs formalin fixed tissue

From:"Brand, Karl" <>


I have been refining a DIG-ISH protocol on formalin fixed, paraffin embedded
tissue over many years and have excellent results thus far.

I am now considering applying this protocol to methacarn fixed tissue.

Any suggestions in simplifying this transition would be greatly appreciated

Ultimately, parity in ISH staining between the two differently fixed
specimens is the goal.



Karl Brand <>
Tissue Array Facility
The Peter MacCallum Cancer Institute
Centre for Cancer Genomics and Predictive Medicine
St Andrews Place, East Melbourne, Australia, 3002
Ph: +61 3 9656 1937, Fax: +61 3 9656 1460, Mob: +61 (0) 400 304503

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