Sucrose as a cryoprotectant

Hi, all histonetters

What is the rationale for using sucrose as a
cryoprotectant. For cryostat sectioning of rat brain
we use graded concentrations of sucrose (15%--> 20-->
30%) for cryoprotection. What is the advantage of
graded concentrations as opposed to immersing the
tissue directly in sucrose.
Hope it merits an answer.

sunil thomas K

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