Re: tartrate-resistant acid phosphatase (TRAP)

From:Roger Moretz <>


Having just done this, I received a couple of very
good responses from the histonet.  One protocol came
from Hermina Borgerink, specifically modified for
osteoclasts, but I used it successfully for
histiocytes.  The reference she included was from J
Cell Biol 132: 195-210, 1996 (Erlebacher & Derynck). 
I also received a paper from a colleague in-house:  J
Histochem Cytochem 48: 219-227, 2000, Hayman, et al. 
Hope these help.  (P.S._check the archives for
Hermina's email--don't have it handy just now.)

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

--- "Kowsz, Kim P" <>
> I am looking for methods and/or comments regarding
> the tartrate-resistant
> acid phosphatase (TRAP) stain as a marker of bone
> resorption (stains
> osteoclasts).  Has anyone used this method on
> formalin fixed, formic acid
> decalcified rat bone?
> Kim Kowsz
> Pfizer Global R & D

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