Re: soaking blocks

From:Otis Lyght <lyght@ciit.org>

Hi All,
Seems there is lots of discussion of "block soaking". I work in a lab that=20
deals with animal tissue. We soak  liver blocks sometimes for up to 2 hours=20
and tissues sometimes still appears to be dry when mounted on the slide. 
Would anyone who is soaking or not soaking share their tissue processing 
schedule. Our total processing time runs approx. 7 hours for mice and 8 
hours for rat (three hours of this is 3 paraffin changes with the remainder=20
being dehydrating and clearing). Would love to get some feedback on this.
Thanks.
Otis Lyght





At 08:10 AM 3/15/01 -0700, you wrote:
>Renee,
>
>you soak your blocks for 10-15 MINUTES????  wow.  I work with vet stuff,
>too and find that only the livers, spleens and sometimes kidneys (if
>there is a lot of blood in them) are about the only tissues I soak.  And
>I only soak for 10-30 SECONDS.  I try to minimize soaking, but for
>bloody, friable tissues, it can't be helped.
>
>Connie M
>
>Renee Escalona wrote:
> >
> > I'll have to admit, soaking blocks over night on ice does seem a little=20
> excessive.   I work in a veterinary lab so of course all we do is process=20
> and cut animal tissue.  I would say that on average we let our blocks 
> soak and chill for about 10-15 minutes.
> >
> > Renee Escalona, BS, HTL(ASCP)
> > Diagnostic Laboratory Supervisor
> > Texas Veterinary Medical Diagnostic Lab
> > College Station, TX
>
>--
>ࡱ




<< Previous Message | Next Message >>