Hi Martha,
    DAKO has a good antibody (M3559). Our dilution is 1:200. We pretreat
with a citrate buffer in a steamer for 20 min. Our detection kit is LSAB+,
but I would assume with a little tweaking of the dilution you could get
just as good results on the Ventana.
Amos Brooks

Martha Ward wrote:

> Martha Ward writes:
>
> I wonder if someone could share their procedure, vendor, etc. for
> myogenin?  At one point we had this working but all of a sudden it has
> quit.  We need a procedure that can be performed on a Ventana ES.
> Thanks in advance for your help!