Re: methyl green is methyl blue
|From:||firstname.lastname@example.org (Teri Johnson)|
There is methyl violet in methyl green that needs to be removed using
chloroform and a separatory funnel. It's a mess to do. I don't remember
where I found the protocol for this, but I'm sure others will be able to
We get around it by buying it commercially prepared.
Physicians Reference Laboratory
Overland Park, KS
----- Original Message -----
From: kwalters <email@example.com>
To: histo <firstname.lastname@example.org>
Sent: Thursday, April 12, 2001 9:45 AM
Subject: methyl green is methyl blue
> Everytime I make methyl green it turns a gorgeous shade of blue. I have a
> brand new powder that I am using.
> Here is my protocol: please tell me where I went wrong!!
> Thanks for any help!!
> Methyl Green
> a ) 0.1 N Acetic Acid: Add 6 ml of glacial acetic acid to 1000 ml of
> filtered distilled water.
> b) 0.1 N sodium Acetate: Add 4.102 g of sodium acetate to 500 ml of
> c) Buffer:
> 1)Add 755 ml of 0.1 N acetic acid to 265 ml of 0.1 N sodium
> 2) Adjust pH to 4.2 with NAOH.
> d) Methyl Green
> 1) Add 20.0 g of methyl green to the buffer solution.
> 2) Filter the methyl green solution.
> STORAGE: room temperature
> SHELF LIFE: Indefinite while in storage; when in use, the shelf
> 2 weeks or until staining becomes too weak.
> Kathy Walters //
> Central Microscopy Research Facility / /
> 85 EMRB / /\
> University of Iowa / /\ \
> Iowa City, Iowa 52242 / / \ \
> Phone #: (319) 335-8142 / / \ \
> Fax #: (319) 384-4469 ______ ((0))
> email: Katherine-Walters@uiowa.edu |__| / /
> || / /
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