Re: cornflaking artifact
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The cornflaking artifact as described by many labs can be solved easily.Our
lab was a victim to it.
Here is the solution, it worked for me.
When you remove the slides from the stainer DO NOT LET THEM AIR DRY,
before coverslipping in the automated coverslipper,keep a fresh dish of
xylene next to the coverslipper and cover slip one rack at a time donot pile
more than one rack on to the coverslipper,and make sure that the rest of the
rack are sitting in xylene while the coverslipper is busy,this will solve
the cornflaking artifact.
If you are using an automated stainer make sure you change the final absolute
alcohol after every three runs.
The way to find out if your alcohol has water in the final step is if your
xylene turns cloudy.The other way is to use a hydrometer .
The cornflaking artifact is due to air being trapped in your tissue and not
water.
Make sure that the flow of xylene from the auto coverslipper is consistent
and at least 8 drops for each slide.
Good luck.
Akbar,
El camino hospital
Mt.View.
California.
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>The cornflaking artifact as described by many labs can be solved easily.Our
<BR>lab was a victim to it.
<BR>Here is the solution, it worked for me.
<BR>When you remove the slides from the stainer DO NOT LET THEM AIR DRY,
<BR>before coverslipping in the automated coverslipper,keep a fresh dish of
<BR>xylene next to the coverslipper and cover slip one rack at a time donot pile
<BR>more than one rack on to the coverslipper,and make sure that the rest of the
<BR>rack are sitting in xylene while the coverslipper is busy,this will solve
<BR>the cornflaking artifact.
<BR>If you are using an automated stainer make sure you change the final absolute
<BR>alcohol after every three runs.
<BR>The way to find out if your alcohol has water in the final step is if your
<BR>xylene turns cloudy.The other way is to use a hydrometer .
<BR>The cornflaking artifact is due to air being trapped in your tissue and not
<BR>water.
<BR>Make sure that the flow of xylene from the auto coverslipper is consistent
<BR>and at least 8 drops for each slide.
<BR>Good luck.
<BR>Akbar,
<BR>El camino hospital
<BR>Mt.View.
<BR>California.</FONT></HTML>
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