On Tue, 3 Apr 2001 AndreaH@imclone.com wrote:

> I am just wondering what people are generally using to block endogenous
> alkaline phosphotase in frozen tissue sections .... ??

I think levamisole or tetramisole (10-3M) [that's ten to the
minus three molar] is usual. An older method was to treat
the section with an iodine - thiosulphate sequence (as if
you had used a fixative containing mercuric chloride). Most
endogenous alkaline phosphatases are inhibited by fluoride
ions, but some neurons in sensory ganglia contain an
enzyme called FRAP that isn't.


----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan