Re: alk phos block

From:"J. A. Kiernan" <>

On Tue, 3 Apr 2001 wrote:

> I am just wondering what people are generally using to block endogenous
> alkaline phosphotase in frozen tissue sections .... ??

I think levamisole or tetramisole (10-3M) [that's ten to the
minus three molar] is usual. An older method was to treat
the section with an iodine - thiosulphate sequence (as if
you had used a fixative containing mercuric chloride). Most
endogenous alkaline phosphatases are inhibited by fluoride
ions, but some neurons in sensory ganglia contain an
enzyme called FRAP that isn't.

John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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