Re: T and B cell markers in Paraffins

From:Katri Tuomala <>

Hi Dave,
I use Dako clone L26 for B cells and Dako polyclonal CD3 for T cells.
L26 does not necessarily require retrieval and for CD3 I use 0.05% pronase E for
15 minutes at room temperature. You'll have to work out the titres to suit your
detection system.

Katri Tuomala
St.Joseph's Health Care
Hamilton, Ontario, Canada

"Kinsley, David" wrote:

> Hi all,
> does anyone know of good markers/antigen retrieval or amplification
> procedures  for T cell and Bcell ID/characterization in formalin fixed
> paraffin embedded tissue?  I can only get them to work in frozens?  CD3,
> CD3e, CD45R, ......any assisitance is appreciated.
> thanks in advance
> Dave Kinsley MS,HT/HTL(ASCP)
> Schering-Plough Research Institute
> Department of Immunology
> 2015 Galloping Hill Road
> Kenilworth, New Jersey 07033
> Phone:  908-740-4669
> Fax:      908-740-3084
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