Re: T and B cell markers in Paraffins
From: | Katri Tuomala <katri@istar.ca> |
Hi Dave,
I use Dako clone L26 for B cells and Dako polyclonal CD3 for T cells.
L26 does not necessarily require retrieval and for CD3 I use 0.05% pronase E for
15 minutes at room temperature. You'll have to work out the titres to suit your
detection system.
Katri
Katri Tuomala
St.Joseph's Health Care
Hamilton, Ontario, Canada
"Kinsley, David" wrote:
> Hi all,
>
> does anyone know of good markers/antigen retrieval or amplification
> procedures for T cell and Bcell ID/characterization in formalin fixed
> paraffin embedded tissue? I can only get them to work in frozens? CD3,
> CD3e, CD45R, ......any assisitance is appreciated.
>
> thanks in advance
>
> Dave Kinsley MS,HT/HTL(ASCP)
>
> Schering-Plough Research Institute
> Department of Immunology
> 2015 Galloping Hill Road
> Kenilworth, New Jersey 07033
>
> Phone: 908-740-4669
> Fax: 908-740-3084
>
> ***************************************************************
> This electronic message, including its attachments, is confidential and
> proprietary and is solely for the intended recipient. If you are not the
> intended recipient, this message was sent to you in error and you are hereby
> advised that any review, disclosure, copying, distribution or use of this
> message or any of the information included in this message by you is
> unauthorized and strictly prohibited. If you have received this electronic
> transmission in error, please immediately notify the sender by reply to this
> message and permanently delete all copies of this message and its
> attachments in your possession. Thank you.
<< Previous Message | Next Message >>