I assume that the use of graded sucrose solutions is to prevent suddent
osmotic changes which could disrupt cells and tissue.
Barry

sthomaska@yahoo.com wrote:

> Hi, all histonetters
>
> What is the rationale for using sucrose as a
> cryoprotectant. For cryostat sectioning of rat brain
> we use graded concentrations of sucrose (15%--> 20-->
> 30%) for cryoprotection. What is the advantage of
> graded concentrations as opposed to immersing the
> tissue directly in sucrose.
> Hope it merits an answer.
>
> sunil thomas K
>
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