Re: PLP fix
From: | Connie McManus <conmac@cc.usu.edu> |
Which Lysine, D or L form, do you use --- or does it matter?
Connie M
Joan Yonchek wrote:
>
> Carrie,
>
> PLP stands for Periodate-Lysine-Paraformaldehyde.
>
> See: McLean IW and Nakane PK J Histochem Cytochem 22:1077-1083, 1974.
>
> PLP Working Solution
>
> 50 ml 3% (w/v) Paraformaldehyde
> 100ml 0.1 M Disodium hydrogen othophosphate
> 0.9g Lysine
> 0.15g Sodium periodate
>
> Adjust to pH 7.4
>
> Joan
> RTI
>
> ------------------------------------------------------------------------
>
> Subject: PLP fix
> Date: Tue, 10 Apr 2001 10:12:33 -0400
> From: "Cartwright, Carrie" <carrie.cartwright@Agouron.COM>
> To: "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
>
> Can anyone tell me what PLP stands for for PLP fixative?
> Thanks,
> Carrie
--
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