Re: PLP fix

From:Connie McManus <conmac@cc.usu.edu>

Which Lysine, D or L form, do you use --- or does it matter?

Connie M

Joan Yonchek wrote:
> 
> Carrie,
> 
> PLP stands for Periodate-Lysine-Paraformaldehyde.
> 
> See:  McLean IW and Nakane PK  J Histochem Cytochem 22:1077-1083, 1974.
> 
> PLP Working Solution
> 
>  50 ml      3% (w/v) Paraformaldehyde
> 100ml      0.1 M Disodium hydrogen othophosphate
>  0.9g       Lysine
> 0.15g      Sodium periodate
> 
> Adjust to pH 7.4
> 
> Joan
> RTI
> 
>   ------------------------------------------------------------------------
> 
> Subject: PLP fix
> Date: Tue, 10 Apr 2001 10:12:33 -0400
> From: "Cartwright, Carrie" <carrie.cartwright@Agouron.COM>
> To: "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
> 
> Can anyone tell me what PLP stands for for PLP fixative?
> Thanks,
> Carrie

-- 
ࡱ



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