Re: Daily Digest

From:Jeff Silverman <peptolab@hamptons.com>

Dan I currently run a Cytologix Artisan and have demoed the Nexus in the
past. The Artisan has a smaller footprint on the bench, produces less waste
than the Nexus -which has some kind of mineral oil in the wash buffer to
prevent slides drying out, and the Artisan separates the waste into four
different bottles for ease of disposal. Of course, you can run immunos and
regular special stains on the same module with the Artisan. I also like the
stains much better on the Artisan- I don't care for the methyl green
counterstaining on the Nexus. Just my two cents. I inherited the Artisan so
I never did cost analysis. Cytologix  support has been excellent.
Jeff Silverman
----- Original Message -----
From: "HistoNet Server" <histonet@pathology.swmed.edu>
To: "HistoNet Server" <histonet@pathology.swmed.edu>
Sent: Friday, April 06, 2001 2:42 AM
Subject: Daily Digest


>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 05:59:20 -0600
> From: Greg Dobbin <dobbin@Upei.CA>
> Subject: Re: IHC decreased staining
>
> Hi Michelle,
> 2 other possibilities for source of problems are the DAB itself
> and/or the H202. Try a new lot of DAB in case there was a problem
> of storage conditions that you may not be aware of (for instance,
> someone left it out on the bench overnight by mistake, and
> neglected to mention it to you). And I understand (from this list at
> one point, a long time ago), that hydrogen peroxide can go "bad",
> after long term storage, something about forming water molecules.
> I'm sure someone else out there has the proper explanation.
> Good luck.
> Greg
>
> Date sent:      Wed, 04 Apr 2001 16:11:08 -0400
> From:           Michelle Peiffer <mlk101@psu.edu>
> Subject:        IHC decreased staining
> Forwarded to:   DOBBIN@acad1.cs.upei.ca
> To:             "histonet@pathology.swmed.edu"
<histonet@pathology.swmed.edu>
>
> > Hi all,
> >
> > We are have major problems with our IHC and I'm hoping someone out there
> > may have some insight.  Basically the staining intensity has decreased;
> > within the same block the DAB is about half as dark as it was 2 months
ago,
> > using the same protocol.  We are using the DAKO autostainer, and DAKO
kits
> > (incidently their tech support is working with me on this too)
> >
> > I've  tested 6 different antibodies, using 4 different DAKO kits.  I've
> > stained in the autostainer and by hand.  I've tested the dewaxing agents
> > (histosolve, xylene, and another xylene substitute) and 2 different
methods
> > of baking the sections onto the slides.  In every case the intensity of
DAB
> > staining is lighter, about 1/2 of the intensity as done previously on
the
> > same blocks.
> >
> > I've checked the pH of our water used in the buffer.  Strangely our
> > distilled water pH is 9.1 and our deionized water in pH 9.3.  Though the
> > buffer itself is 7.5. DAKO is checking to see what effect this may have,
it
> > is definately not what is recommended.
> >
> > Any other suggestions?  Thanks for your advice,
> >
> > Michelle
> >
> >
> >
> > Michelle Peiffer
> > *************************************************************
> > Electron Microscope Facility for the Life Sciences
> > Penn State University Biotechnology Institute
> > 001 South Frear Lab
> > University Park PA 16802
> >
> > phone: 814-865-0212
> > email:  mlk101@psu.edu
> > **************************************************************
> >
> >
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 07:11:16 -0600
> From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
> Subject: Revalidating immuno reagents
>
> Good morning all,
> During the last two days, we have been inspected by CLIA. The
> inspector found an expired immuno reagent in the refrigerator (I know,
shame
> on me).  I explained to this person that although the reagent is expired,
I
> always run a known positive control with each run.  If the control didn't
> work, the case is repeated with another lot number. This was not good
enough
> for this person. She wanted proof.  Short of performing a short immuno run
> in front of this person, I couldn't site any references.
> Does any one have a procedure that can be referenced dealing with
> validaing and re-validating reagents?  I'll also mention that each primary
> antibody gets titered upon receipt and each new detection kit gets tested
> and the results recorded before it is put into use.
> Thanks in advance.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 07:11:34 -0600
> From: ANATECH LTD <email@anatechltdusa.com>
> Subject: Transfer fixative
>
> Ann Maruska asked about a fixative/transfer fluid that would hold
> specimens for weeks without loss of immunoreactivity.  Zinc formalin
> in most of its formulations will do that.
>
> Dick
>
> - --
> Richard W. Dapson, Ph.D.
> Anatech Ltd.
> Battle Creek, MI
> 800-ANATECH (800-262-8324)
> email@anatechltdusa.com
> Web address:  anatechltdusa.com
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 08:02:05 -0600
> From: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
> Subject: RE: IHC decreased staining
>
> Michelle,
>
> Are you cutting fresh slides each time you stain or are these precut
slides
> that may be losing antigenicity upon storage?
>
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-2472
> 600 Highland Ave.
> Madison, WI 53792-2472
> (608)265-6596
> FAX: (608)262-7174
>
>
> - -----Original Message-----
> From: Michelle Peiffer [mailto:mlk101@psu.edu]
> Sent: Wednesday, April 04, 2001 3:11 PM
> To: histonet@pathology.swmed.edu
> Subject: IHC decreased staining
>
>
> Hi all,
>
> We are have major problems with our IHC and I'm hoping someone out there
> may have some insight.  Basically the staining intensity has decreased;
> within the same block the DAB is about half as dark as it was 2 months
ago,
> using the same protocol.  We are using the DAKO autostainer, and DAKO kits
> (incidently their tech support is working with me on this too)
>
> I've  tested 6 different antibodies, using 4 different DAKO kits.  I've
> stained in the autostainer and by hand.  I've tested the dewaxing agents
> (histosolve, xylene, and another xylene substitute) and 2 different
methods
> of baking the sections onto the slides.  In every case the intensity of
DAB
> staining is lighter, about 1/2 of the intensity as done previously on the
> same blocks.
>
> I've checked the pH of our water used in the buffer.  Strangely our
> distilled water pH is 9.1 and our deionized water in pH 9.3.  Though the
> buffer itself is 7.5. DAKO is checking to see what effect this may have,
it
> is definately not what is recommended.
>
> Any other suggestions?  Thanks for your advice,
>
> Michelle
>
>
>
> Michelle Peiffer
> *************************************************************
> Electron Microscope Facility for the Life Sciences
> Penn State University Biotechnology Institute
> 001 South Frear Lab
> University Park PA 16802
>
> phone: 814-865-0212
> email:  mlk101@psu.edu
> **************************************************************
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 08:02:41 -0600
> From: STYLER@dnr.state.md.us
> Subject: rickettsia controls
>
> I am searching for a rickettsia control block or slides. Does anyone out
> there have a source?
>
> Thank you. Sue Tyler HT (ASCP)
>
> Fisheries Service
> 904 South Morris Street
> Oxford, MD 21654
> styler@dnr.state.md.us
>
> ###########################################
>
> This message has been scanned for viruses.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 08:02:56 -0600
> From: "Morken, Tim" <tim9@cdc.gov>
> Subject: RE: Revalidating immuno reagents
>
> Joe, CLIA was satisfied with our records showing previous uses of
> antibodies. I developed a database that keeps track of all experiments and
> can collate all uses of any antibody into an "antibody information sheet."
> This sheet shows all information about a particular antibody, including
all
> previous uses of that antibody, slide by slide, and the results of those
> uses, including comments about performance.
>
> Tim Morken, BA, EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology Activity
> Centers for Disease Control and Prevention
> Ms-G32
> 1600 Clifton Road
> Atlanta, GA 30333
> USA
>
> PH: 404-639-3964
> FAX: 404-639-3043
>
> email: tim9@cdc.gov
>
>
>
> - -----Original Message-----
> From: Nocito, Joseph [mailto:joseph_nocito@srhc.iwhs.org]
> Sent: Thursday, April 05, 2001 8:38 AM
> To: 'Histonet'
> Subject: Revalidating immuno reagents
>
>
> Good morning all,
> During the last two days, we have been inspected by CLIA. The
> inspector found an expired immuno reagent in the refrigerator (I know,
shame
> on me).  I explained to this person that although the reagent is expired,
I
> always run a known positive control with each run.  If the control didn't
> work, the case is repeated with another lot number. This was not good
enough
> for this person. She wanted proof.  Short of performing a short immuno run
> in front of this person, I couldn't site any references.
> Does any one have a procedure that can be referenced dealing with
> validaing and re-validating reagents?  I'll also mention that each primary
> antibody gets titered upon receipt and each new detection kit gets tested
> and the results recorded before it is put into use.
> Thanks in advance.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:00:08 -0600
> From: "Mary Bryhan" <mbryhan@northernhealth.org>
> Subject: RE: IHC decreased staining
>
> To add to what Dave said, most of the antibody vendors will work with you
on
> which antibodies are most vulnerable to antigen loss.  Good luck!
>
>
> Mary Bryhan HT (ASCP)
> Northern Michigan Hospital
> Petoskey, Michigan
>
> >>> David Reynolds <DReynolds@SNBLUSA.com> 04/04/01 06:51PM >>>
> Hi - I am unaware of how your control slides and tissues are treated, you
> may have already thought of this.
>
> There may be some antigenicity lost due to oxidation of your tissue.  If
the
> control slides are cut well in advance, it would be good to keep them
> refrigerated until they are used.  Zymed had a list of antigens that were
> particularly vulnerable to this, while I do not have the list in front of
me
> I do remember that cytokeratins and ER/PR were among them.  I am sure
there
> will be many other suggestions to follow from the real experts, I am
> currently just a dabbler in IHC.
>
> Good luck
>
> David A. Reynolds, HT(ASCP)
> SNBL USA, Ltd.
> 6605 Merrill Creek Parkway
> Everett, WA  98203
>
>
> Confidentiality Notice: This email, its contents and attachments are
> confidential and may contain privileged information. It is intended solely
> for the use of addressee(s) only. Any use, copying or disclosure of this
> communication or attachments to any other person is expressly prohibited
> without written permission of SNBL-USA,Ltd. If you receive this message in
> error, please notify the sender at SNBL USA, Ltd. immediately by return
> e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate
> your cooperation.
>
>
>
>
> - -----Original Message-----
> From: Michelle Peiffer [mailto:mlk101@psu.edu]
> Sent: Wednesday, April 04, 2001 1:11 PM
> To: histonet@pathology.swmed.edu
> Subject: IHC decreased staining
>
>
> Hi all,
>
> We are have major problems with our IHC and I'm hoping someone out there
> may have some insight.  Basically the staining intensity has decreased;
> within the same block the DAB is about half as dark as it was 2 months
ago,
> using the same protocol.  We are using the DAKO autostainer, and DAKO kits
> (incidently their tech support is working with me on this too)
>
> I've  tested 6 different antibodies, using 4 different DAKO kits.  I've
> stained in the autostainer and by hand.  I've tested the dewaxing agents
> (histosolve, xylene, and another xylene substitute) and 2 different
methods
> of baking the sections onto the slides.  In every case the intensity of
DAB
> staining is lighter, about 1/2 of the intensity as done previously on the
> same blocks.
>
> I've checked the pH of our water used in the buffer.  Strangely our
> distilled water pH is 9.1 and our deionized water in pH 9.3.  Though the
> buffer itself is 7.5. DAKO is checking to see what effect this may have,
it
> is definately not what is recommended.
>
> Any other suggestions?  Thanks for your advice,
>
> Michelle
>
>
>
> Michelle Peiffer
> *************************************************************
> Electron Microscope Facility for the Life Sciences
> Penn State University Biotechnology Institute
> 001 South Frear Lab
> University Park PA 16802
>
> phone: 814-865-0212
> email:  mlk101@psu.edu
> **************************************************************
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:00:21 -0600
> From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
> Subject: Re: transfer fixative
>
> Ann
> I would be wary of leaving tissue in most fixing or transfer solutions
that
> long.
> There was a study done in the 50s in which portions of brain tissue
collected
> in the field (believe that this was in Africa) were stored in
> 50% glycerin. The tissue could be left in this solution and autolytic and
> bacterial decomposition were  prevented. When required the tissue
> was then placed in fixative. This technique permitted multiple samples to
be
> collected  under field conditions and then examined when back at
> the lab. If my memory serves me correctly,  the study was looking at Negri
> bodies and was published in the Journal of the Institute of
> Medical Laboratory Technology in England. I have  used this technique to
> transport samples to another investigator who wanted to carry out
> IHC using frozen sections. The histology was not as quite as good as with
the
> standard method of freezing or of fixing and then cutting
> frozens but did provide the information we wanted.
> Hope that this helps.
> Barry
>
> ANN MARUSKA wrote:
>
> > Hi histonetters,
> >
> > I have a researcher who will be collecting specimens world wide for an
HIV
> study.  Many of these will be coming from 3rd world countries.
> > My question is:  Is there a fixative that these can be collected in and
> remain for 4-6 weeks before Immunostains are performed on them?
> > Thanks in advance!
> >
> > Ann Maruska
> > Fairview-University Medical Center
> > Mpls. MN  55454
> > amarusk1@fairview.org
> > 612-273-9119
> >
>
  ------------------------------------------------------------------------
> >
> >    ANN MARUSKA.vcfName: ANN MARUSKA.vcf
> >                   Type: Plain Text (text/plain)
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:28:43 -0600
> From: Rebecca S Smith <bssvpisu@iastate.edu>
> Subject: soakers
>
> Just curious!  Are we all talking about soaking with just good ole H2O?
We
> have 3-4 different things we soak in besides the usual water.  For the
> bloodier specimens we find that a weak solution (1-5%) of ammonia
hydroxide
> is helpful.  This particular solution is quite helpful on cartilagenous
> tissues also.  We have a "fibrous soak" and a decal 'block surface' soaker
> too.  Just thought I'd add my 2 cents and a little variety to our lives.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:28:58 -0600
> From: Michelle Peiffer <mlk101@psu.edu>
> Subject: storing controls
>
> Thanks to all who responded about my IHC decreased staining post.
>
> Several people have mentioned storage of the controls.  Some are purchased
> slides, so obviously they are precut and they are stored in the
> refrigerator.  But most are freshly cut from blocks which are stored at
> room temperature.  I was under the impression most people store blocks at
> room temperature, is this correct?  How long can controls last?  We just
> started doing histology so our oldest blocks are only 6 months old.
>
>
> Michelle
>
>
> Michelle Peiffer
> *************************************************************
> Electron Microscope Facility for the Life Sciences
> Penn State University Biotechnology Institute
> 001 South Frear Lab
> University Park PA 16802
>
> phone: 814-865-0212
> email:  mlk101@psu.edu
> **************************************************************
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:29:16 -0600
> From: "Histo-Scientific Research Laboratories" <histosci@shentel.net>
> Subject: Re: Securline Marker II/Superfrost Pens
>
> Dear Richard,
>
> Yes, we have been having terrible problems with the Secureline II
> markers-even with our normal H+E stainer.  Unfortunately, Precision
Dynamics
> Corp. have not returned our call.  Good luck.
>
> - -Beth Poole
> HSRL
> www.hsrl.org
> beth@hsrl.org
> 137 South Main Street
> Woodstock, VA  22664
> (540)459-8211
> fax: (540)459-8211
> - ----- Original Message -----
> From: "Richard Cartun" <Rcartun@harthosp.org>
> To: <Histonet@pathology.swmed.edu>
> Sent: Wednesday, April 04, 2001 4:15 PM
> Subject: Securline Marker II/Superfrost Pens
>
>
> > I think this problem was discussed previously, but we continue to have
> problems with the Securline Marker II/Superfrost pens that we use to label
> glass slides.  The ink (black & red) does not hold up following
> immunohistochemical staining.  Is anyone else experiencing this problem?
I
> contacted the company  (Precision Dynamics Corporation) today, but have
not
> heard back from them.
> >
> > R. Cartun
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:29:39 -0600
> From: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
> Subject: FW: Revalidating immuno reagents
>
>
>
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-2472
> 600 Highland Ave.
> Madison, WI 53792-2472
> (608)265-6596
> FAX: (608)262-7174
>
>
> - -----Original Message-----
> From: Morken, Tim [mailto:tim9@cdc.gov]
> Sent: Thursday, April 05, 2001 9:39 AM
> To: 'Sebree Linda A.'
> Subject: RE: Revalidating immuno reagents
>
>
> Linda,
> I used to do this sort of thing to satisfy CAP. The difference was that as
> the expiration date for a particular antibody came up we would document
> specifically that that antibody worked at that time and then we would set
a
> new expiration date of 6 months to one year, depending on what you are
> comfortable with. That satisfied them in the last CAP inspection I went
> through in 1997. CLIA is a bit different, and we are different here at
CDC,
> where most of our antibodies are made in-house, and have no a official
> expiration dates.
>
> Tim
>
> BTW, post this to histonet if you want. I think others would be interested
> in commenting.
>
> - -----Original Message-----
> From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu]
> Sent: Thursday, April 05, 2001 10:32 AM
> To: 'Morken, Tim'
> Subject: RE: Revalidating immuno reagents
>
>
> Tim,
>
> Do you think this type of documentation would satisfy CAP?  Their policy
is
> sort of "don't ask, don't tell" but even so our inhouse QA/QC people
enforce
> CAP regulations by the book so they've never let us use expired reagents.
>
> Linda A. Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-2472
> 600 Highland Ave.
> Madison, WI 53792-2472
> (608)265-6596
> FAX: (608)262-7174
>
>
> - -----Original Message-----
> From: Morken, Tim [mailto:tim9@cdc.gov]
> Sent: Thursday, April 05, 2001 8:36 AM
> To: 'Histonet'
> Subject: RE: Revalidating immuno reagents
>
>
> Joe, CLIA was satisfied with our records showing previous uses of
> antibodies. I developed a database that keeps track of all experiments and
> can collate all uses of any antibody into an "antibody information sheet."
> This sheet shows all information about a particular antibody, including
all
> previous uses of that antibody, slide by slide, and the results of those
> uses, including comments about performance.
>
> Tim Morken, BA, EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology Activity
> Centers for Disease Control and Prevention
> Ms-G32
> 1600 Clifton Road
> Atlanta, GA 30333
> USA
>
> PH: 404-639-3964
> FAX: 404-639-3043
>
> email: tim9@cdc.gov
>
>
>
> - -----Original Message-----
> From: Nocito, Joseph [mailto:joseph_nocito@srhc.iwhs.org]
> Sent: Thursday, April 05, 2001 8:38 AM
> To: 'Histonet'
> Subject: Revalidating immuno reagents
>
>
> Good morning all,
> During the last two days, we have been inspected by CLIA. The
> inspector found an expired immuno reagent in the refrigerator (I know,
shame
> on me).  I explained to this person that although the reagent is expired,
I
> always run a known positive control with each run.  If the control didn't
> work, the case is repeated with another lot number. This was not good
enough
> for this person. She wanted proof.  Short of performing a short immuno run
> in front of this person, I couldn't site any references.
> Does any one have a procedure that can be referenced dealing with
> validaing and re-validating reagents?  I'll also mention that each primary
> antibody gets titered upon receipt and each new detection kit gets tested
> and the results recorded before it is put into use.
> Thanks in advance.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:29:55 -0600
> From: "Morken, Tim" <tim9@cdc.gov>
> Subject: Database examples:  Revalidating immuno reagents
>
> I have had a lot of people ask for an example of the antibody info sheet I
> mentioned (below). I will send printouts via email to those people, but I
> thought anyone interested in that sort of thing would be interested in the
> workshop I will be giving at the NSH convention in September. It will
cover
> developing databases in MS-Access and will specifically cover applications
> to IHC and other lab testing and documentation. This will be an advanced
> class and will assume you have used Access before.
>
>
> Tim
>
>
> - -----Original Message-----
> From: Morken, Tim [mailto:tim9@cdc.gov]
> Sent: Thursday, April 05, 2001 9:36 AM
> To: 'Histonet'
> Subject: RE: Revalidating immuno reagents
>
>
> Joe, CLIA was satisfied with our records showing previous uses of
> antibodies. I developed a database that keeps track of all experiments and
> can collate all uses of any antibody into an "antibody information sheet."
> This sheet shows all information about a particular antibody, including
all
> previous uses of that antibody, slide by slide, and the results of those
> uses, including comments about performance.
>
> Tim Morken, BA, EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology Activity
> Centers for Disease Control and Prevention
> Ms-G32
> 1600 Clifton Road
> Atlanta, GA 30333
> USA
>
> PH: 404-639-3964
> FAX: 404-639-3043
>
> email: tim9@cdc.gov
>
>
>
> - -----Original Message-----
> From: Nocito, Joseph [mailto:joseph_nocito@srhc.iwhs.org]
> Sent: Thursday, April 05, 2001 8:38 AM
> To: 'Histonet'
> Subject: Revalidating immuno reagents
>
>
> Good morning all,
> During the last two days, we have been inspected by CLIA. The
> inspector found an expired immuno reagent in the refrigerator (I know,
shame
> on me).  I explained to this person that although the reagent is expired,
I
> always run a known positive control with each run.  If the control didn't
> work, the case is repeated with another lot number. This was not good
enough
> for this person. She wanted proof.  Short of performing a short immuno run
> in front of this person, I couldn't site any references.
> Does any one have a procedure that can be referenced dealing with
> validaing and re-validating reagents?  I'll also mention that each primary
> antibody gets titered upon receipt and each new detection kit gets tested
> and the results recorded before it is put into use.
> Thanks in advance.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:55:29 -0600
> From: JanMinshew@aol.com
> Subject: Re: soaking blocks
>
>
> Otis,
>
> You will see an amazing difference in your tissue specimens if you process
in
> a laboratory microwave instead of a routine tissue processor.  Using
> microwave processing procedures the exposure times in alcohol and paraffin
> are greatly reduced and clearants are not used at all.  The tissues are
> normally much easier to section and in most cases you won't need to soak
> them.
>
> Laboratory microwaves suitable for processing are distributed by several
> different manufacturers (Thermo-Shandon, Richard Allan Scientific, Hacker
> Instruments and TBS, Inc).  I would be happy to answer any questions you
> might have.
>
> Jan Minshew
> Technical Director
> TBS, Inc.
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
>
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> Content-Type: text/plain; charset="US-ASCII"
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>
> - --part1_9b.133ba82b.27fde496_boundary
> Content-Type: text/html; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
> <HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Otis,
> <BR>
> <BR>You will see an amazing difference in your tissue specimens if you
process
> in
> <BR>a laboratory microwave instead of a routine tissue processor.
 Using
> <BR>microwave processing procedures the exposure times in alcohol and
paraffin
>
> <BR>are greatly reduced and clearants are not used at all.  The
tissues
> are
> <BR>normally much easier to section and in most cases you won't need to
soak
> <BR>them.
> <BR>
> <BR>Laboratory microwaves suitable for processing are distributed by
several
> <BR>different manufacturers (Thermo-Shandon, Richard Allan Scientific,
Hacker
> <BR>Instruments and TBS, Inc).  I would be happy to answer any
questions
> you
> <BR>might have.
> <BR>
> <BR>Jan Minshew
> <BR>Technical Director
> <BR>TBS, Inc. </FONT></HTML>
>
> - --part1_9b.133ba82b.27fde496_boundary--
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 09:55:47 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: RE: Revalidating immuno reagents
>
> Maybe the problem is that you did not document that you extended the
> expiration date. It's the 'ole if you don't document you took the
> temperature, you didn't take the temp - even if you did.
>
> Below is our policy. I have never been inspected by CLIA only CAP and the
> State, and am a CAP inspector myself.
>
> Marg
>
> Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
> Supervisor, Anatomic Pathology
> Eisenhower Medical Center
> 39-000 Bob Hope Drive
> Rancho Mirage, CA 92270
>
> (It is necessary to mark on the antibody itself that it is extended and to
> what date.)
>
> In an effort to contain costs without compromising quality of performance,
> the expiration dates may be extended on certain immunoreagents. These
> reagents often perform with excellent results up to a year after the
> expiration date. The reactivity will be monitored closely and use
> discontinued if any change/difference in performance is observed.
Expiration
> dates will not be extended longer than three months at a time. (At three
> months if the reagent is still performing optimally, the date is extended
> another 3 months and the extension date is changed on the reagent and the
> change recorded.) All immunohistochemical stains are checked/evaluated and
> reactivity recorded each time a procedure is done. If a reagent with an
> extended expiration date is not performing up to our usual standards, the
> reagent(s) will be discarded and replaced immediately. Any procedure done
> with substandard reagents will be repeated and corrective actions
recorded.
> Any reagent used beyond the expiration date will be marked with the
extended
> date and recorded on the, "IMMUNOREAGENTS EXTENDED EXPIRATION" worksheet.
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 11:43:32 -0600
> From: Barbara.Davies@memhospcs.org
> Subject: Re: Formaldehyde neutralizing vs recycling
>
>
> Teri,
>
> We have been recycling our formalin for years.  We do not have any
> additional exposure.  We empty the formalin into the container that gets
> placed onto the recycler.  The recycler recycles it into the same carboy
> that we place on the shelf and dispense from.   We do have ventilation on
> the actual recycler.   WE still have to purchase half of our formalin but
> it has been very successful.
>
>
> Barb Davies
> Memorial Hospital
> Colorado Springs, CO
>
>
>
> terij@prlnet.com (Teri Johnson)
>
>
> terij@prlnet.com (Teri Johnson) on 04/04/2001 10:11:24 AM
>
> To:   histonet <histonet@pathology.swmed.edu>
> cc:
>
> Subject:  Formaldehyde neutralizing vs recycling
>
>
> Does anybody have any information or bias regarding formalin treatment
> prior
> to disposal or recycling?  I'd like to hear from some places who are
> treating their formalin before disposing of it, and from others who are
> recycling it.  It seems expensive to neutralize the formalin, but one has
> to
> weigh that against collection and handling formalin (testing and
> re-buffering, not to mention formaldehyde exposure with loading and
> unloading a recycling machine).  It seems that for the long run, recycling
> is the best bang for the buck, but I do have concerns about increasing our
> exposure levels with all that handling.
>
> Thanks for your input!
>
> - -Teri Johnson
> Physicians Reference Laboratory
> Overland Park, KS
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 11:43:47 -0600
> From: Paul Shore <p.shore@laboratories.fsnet.co.uk>
> Subject: RE Myogenin
>
> Novocastra supply an antibody to myogenin ( NCL-Myf-4) which is
> effective on the Ventana Nexes so should work on the ES. Unfortunately
> to obtain reliable results an amplification is required. WE  would
> recommend  NCL-Myf-4 1/10, 16 mins incubation. Basic DAB kit.
> Amplification. Data sheets and distributor details can be found on our
> web site www.novocastra.co.uk
>
>
> Paul Shore
> Technical Services
> Novocastra Laboratories
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:11:40 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: RE: soaking blocks
>
> Bravo, Jan, for including all manufacturers.
>
> Marg
>
>
>
> - -----Original Message-----
> From: JanMinshew@aol.com [mailto:JanMinshew@aol.com]
> Sent: Thursday, April 05, 2001 8:09 AM
> To: lyght@ciit.org; conmac@cc.usu.edu
> Cc: histonet@pathology.swmed.edu
> Subject: Re: soaking blocks
>
>
> Otis,
>
> You will see an amazing difference in your tissue specimens if you process
> in
> a laboratory microwave instead of a routine tissue processor.  Using
> microwave processing procedures the exposure times in alcohol and paraffin
> are greatly reduced and clearants are not used at all.  The tissues are
> normally much easier to section and in most cases you won't need to soak
> them.
>
> Laboratory microwaves suitable for processing are distributed by several
> different manufacturers (Thermo-Shandon, Richard Allan Scientific, Hacker
> Instruments and TBS, Inc).  I would be happy to answer any questions you
> might have.
>
> Jan Minshew
> Technical Director
> TBS, Inc.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:12:10 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: RE: storing controls
>
> Michelle,
>
> I am not sure how long controls last, I have not done a scientific study
as
> I am sure some have. Through experience we have found that paraffin
blocks,
> even unsealed, seem to retain antigenicity for a very, very long time.
> Years. As for cut control slides - for some antigens (HER2) cut sections
can
> remain at room temperature for just a matter of days before the
antigenicity
> starts to decline. Immuno control slides deteriorate faster if they have
> been incubated or stored at room temperature. From my experience, I would
> recommend that controls slides be cut, but not incubated, and stored in
the
> refrigerator under dry conditions. The expiration on these slides is up
for
> grabs, I would be interested in what others have to say. We go through our
> breast marker controls very rapidly and all others have a 1 year
expiration
> after they are cut. However, we go through the controls in a matter of a
few
> months.
>
> Good luck,
> Marg
>
> Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
> Supervisor, Anatomic Pathology
> Eisenhower Medical Center
> 39-000 Bob Hope Drive
> Rancho Mirage, CA 92270
>
>
> Marg
>
>
> - -----Original Message-----
> From: Michelle Peiffer [mailto:mlk101@psu.edu]
> Sent: Thursday, April 05, 2001 7:29 AM
> To: histonet@pathology.swmed.edu
> Subject: storing controls
>
>
> Thanks to all who responded about my IHC decreased staining post.
>
> Several people have mentioned storage of the controls.  Some are purchased
> slides, so obviously they are precut and they are stored in the
> refrigerator.  But most are freshly cut from blocks which are stored at
> room temperature.  I was under the impression most people store blocks at
> room temperature, is this correct?  How long can controls last?  We just
> started doing histology so our oldest blocks are only 6 months old.
>
>
> Michelle
>
>
> Michelle Peiffer
> *************************************************************
> Electron Microscope Facility for the Life Sciences
> Penn State University Biotechnology Institute
> 001 South Frear Lab
> University Park PA 16802
>
> phone: 814-865-0212
> email:  mlk101@psu.edu
> **************************************************************
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:12:27 -0600
> From: Barbara.Davies@memhospcs.org
> Subject: Re: Revalidating immuno reagents
>
>
> We put a note on each 'expired' antibody that it 'requires re-validation'.
> Then when we re-validate (run it again with the appropriate control
slides)
> we use our initial validation form, designate a re-validation and document
> the results.  We keep these in a manual, filed under the antibody name
with
> the spec sheets and lot numbers, dates, etc.   I don't know if this would
> help all inspectors but it may be worth a try.
>
> Barb Davies
> Memorial Hospital
> Colorado Springs, CO
>
>
>
> "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
>
>
> "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> on 04/05/2001 06:37:33 AM
>
> To:   'Histonet' <histonet@pathology.swmed.edu>
> cc:
>
> Subject:  Revalidating immuno reagents
>
>
> Good morning all,
>      During the last two days, we have been inspected by CLIA. The
> inspector found an expired immuno reagent in the refrigerator (I know,
> shame
> on me).  I explained to this person that although the reagent is expired,
I
> always run a known positive control with each run.  If the control didn't
> work, the case is repeated with another lot number. This was not good
> enough
> for this person. She wanted proof.  Short of performing a short immuno run
> in front of this person, I couldn't site any references.
>      Does any one have a procedure that can be referenced dealing with
> validaing and re-validating reagents?  I'll also mention that each primary
> antibody gets titered upon receipt and each new detection kit gets tested
> and the results recorded before it is put into use.
>      Thanks in advance.
>
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:12:44 -0600
> From: "George, Cheryl" <CGerorge@Elliot-HS.org>
> Subject: RE: storing controls
>
> Michelle,
>
> We store all of our control blocks and slides in the refridgerator.
>
> Cheryl
>
> > ----------
> > From: Michelle Peiffer[SMTP:mlk101@psu.edu]
> > Sent: Thursday, April 05, 2001 10:28 AM
> > To: histonet@pathology.swmed.edu
> > Subject: storing controls
> >
> > Thanks to all who responded about my IHC decreased staining post.
> >
> > Several people have mentioned storage of the controls.  Some are
purchased
> > slides, so obviously they are precut and they are stored in the
> > refrigerator.  But most are freshly cut from blocks which are stored at
> > room temperature.  I was under the impression most people store blocks
at
> > room temperature, is this correct?  How long can controls last?  We just
> > started doing histology so our oldest blocks are only 6 months old.
> >
> >
> > Michelle
> >
> >
> > Michelle Peiffer
> > *************************************************************
> > Electron Microscope Facility for the Life Sciences
> > Penn State University Biotechnology Institute
> > 001 South Frear Lab
> > University Park PA 16802
> >
> > phone: 814-865-0212
> > email:  mlk101@psu.edu
> > **************************************************************
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:13:01 -0600
> From: "Bartlett, Jeanine" <jqb7@cdc.gov>
> Subject: RE: soakers
>
> All of the above!  I use plain water for routine soaking, but I also use
the
> other options you mentioned when the need presents itself.
>
> Jeanne Bartlett
> CDC-Atlanta
>
> - -----Original Message-----
> From: Rebecca S Smith [mailto:bssvpisu@iastate.edu]
> Sent: Thursday, April 05, 2001 10:24 AM
> To: HistoNet Server
> Subject: soakers
>
>
> Just curious!  Are we all talking about soaking with just good ole H2O?
We
> have 3-4 different things we soak in besides the usual water.  For the
> bloodier specimens we find that a weak solution (1-5%) of ammonia
hydroxide
> is helpful.  This particular solution is quite helpful on cartilagenous
> tissues also.  We have a "fibrous soak" and a decal 'block surface' soaker
> too.  Just thought I'd add my 2 cents and a little variety to our lives.
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:13:28 -0600
> From: tflore@lsuhsc.edu (Flores, Teresa)
> Subject: Johns formar coated grids
>
> I forgot to mention John that we do not use formar coated grids. For
better
> assessment of the renal biopsy we do not mince the sample, either. Please
> see our Web page for more details.
> <pathology.lsuhsc.edu/Pathist/dx_home.htlm> click on M.diagnostic service
> and then on renal biopsy.
> Our diagnostic em lab continues to send poloroid HRLM pictures and TEM B&W
> contact prints with each report.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:22:02 -0600
> From: "Krahn, Daniel" <DKrahn@nmh.org>
> Subject: CytoLogix vs. Venatan NexEs SS
>
> Hi all,
> I was hoping to get some comparison info on the Cytologix automated
> special stainer vs. the Ventana NexEs special stainer.  Any thoughts on
> cost, ease of use, quality, etc?  I would appreciate any help as we look
> into a possible purchase.
>
> Thanks much,
> Dan
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 12:39:25 -0600
> From: John Baker <bakerj@umich.edu>
> Subject: cryosectioning large bone samples
>
>
> Does anyone have any tips that might help me cryosection large bone
> samples?  The tissue is human navicular and cuneiform bones.  They have
> been trimmed to about 1cm thick and are about 2.5cm square. I fixed them
in
> 4% Paraformaldehyde for three days, placed in 10% PVA for 2 days, and snap
> frozen in isopentane in a dryice/alcohol slurry.  I have a Hacker OTF
> cryostat and fixed the tissue so I did not have contamination problems. I
> am taking 7 micron sections using the Instrumedics tape window system. But
> the tissue is not sticking to the slide but coming off with the window.
Is
> there a problem that affect the sections. Right now it just crunches into
> the bone.  Using the same spot on the knife I tested a block of trabecular
> bone and it cut like butter.  Any suggestions from those of you who cut
> bone.  Never having cut human tissues before in my cryostat what
> precautions and cleanup methods might you suggest too if I go with the
> unfixed.
> Thank you for your time,  John
>
>
>
> - ------------------ MIME Information follows ------------------
>
> - --==========01217071==========
> Content-Type: text/plain; charset=us-ascii; format=flowed
> Content-Transfer-Encoding: 7bit
> Content-Disposition: inline
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --==========01217071==========
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>
> ==========01205990==========
> - --==========01217071==========
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>
> ==========01217747==========
> - --==========01217071==========--
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 14:42:42 -0600
> From: "Sinteff, Irene" <ISinteff@cytologix.com>
> Subject: Training position with CytoLogix
>
> CytoLogix Corporation in Cambridge, MA is seeking a certified Histotech
for
> a position as in-house Trainer.  Responsibilities include: training new
and
> existing customer on the use and maintenance of the Artisan Staining
System,
> assisting sales with in-house demonstrations of the Artisan for potential
> customers, coordinating all the different aspects of presenting training
> classes, and continually developing and improving customer training
> materials and presentations.
>
> This position requires HT certification with 5-10 years experience overall
> as a Histotech, Histotechnologist or Applications Support Specialist in a
> histotechnology/pathology related field and experience with staining
> automation.  A BA/BS and teaching or training experience is a plus.
> Histology experience should include routine, special and IHC stains, fixed
> and frozen sections, tissue fixation, microtomy, and antigen retrieval
> techniques.  You should have an aptitude for computers and embrace the
idea
> of staining automation in the modern laboratory.  This position is part of
> our Customer Service group so communication (verbal and written),
> organizational and interpersonal skills are critical requirements as is a
> customer service orientation.   The position may require occasional travel
> to customer sites.
>
> CytoLogix is a growing biotech/medical diagnostics company situated across
> the Charles River from beautiful Boston, MA, a hub for education, cultural
> activities, biotech, and innovation.  A great geographic location gives us
> access to terrific beaches, the mountains, and flavors of New England.
> CytoLogix offers a casual work environment with a dedicated team of
> contributors and a competitive compensation package which includes stock
> options, an excellent benefit package, free parking and access to public
> transportation.
>
> If you qualify for the above position, send us your resume and cover
letter
> with salary requirements by April 15, 2001 to careers@cytologix.com.  We
> will consider relocation for the right candidate.     EOE M/F/D/V
>
> Irene Sinteff
> Contract Recruiter
> CytoLogix Corporation
> 99 Erie Street
> Cambridge, MA  02139
> 617-576-0900  x270
> FAX:   617-576-0088
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 14:59:29 -0600
> From: tflore@lsuhsc.edu (Flores, Teresa)
> Subject: Johns formar coated grids
>
> John, I forgot to mention that we do not use formar coated grids. For
better
> assessment of the renal biopsy we do not mince the sample, either. Please
> see our Web page for more details.
> <pathology.lsuhsc.edu/Pathist/dx_home.htlm> click on M.diagnostic service
> and then on renal biopsy.
> Our diagnostic em lab continues to send poloroid HRLM pictures and TEM B&W
> contact prints with each report.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 14:59:48 -0600
> From: "Laurie Colbert" <laurie.colbert@schs.com>
> Subject: Labeling Control Slides
>
> We batch our special stains and only run one control slide per batch.
> When labeling the control slide we write all of the patient accession
> numbers on the label.  BUT, is it required to include the date on the
> control label???  The date (along with all of the other required info)
> is recorded in our Special Stain/QC Log Book.
>
> Laurie Colbert
> Huntington Memorial Hospital
> Pasadena, CA
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 15:00:11 -0600
> From: KoellingR@immunex.com
> Subject: RE: storing controls
>
> Cheryl,
> Do you keep test (or patient blocks) in refrigerator?  Just curious about
> your thoughts regarding having a control  block/section nicely positive
for
> "X" from the refrigerator stained next to a test section/block for "X"
that
> has been stored at room temp and is negative.  Would it truly be so?
>
> Thanks,
> Ray
> Seattle, WA
>
>
>
>
>                     "George,
>
>                     Cheryl"               To:
> histonet@pathology.swmed.edu, 'Michelle
>                     <CGerorge@Elli        Peiffer' <mlk101@psu.edu>
>
>                     ot-HS.org>            cc:
>
>                                           Subject:     RE: storing
controls
>
>                     04/05/01 09:36
>
>                     AM
>
>
>
>
>
>
>
>
> Michelle,
>
> We store all of our control blocks and slides in the refridgerator.
>
> Cheryl
>
> > ----------
> > From:         Michelle Peiffer[SMTP:mlk101@psu.edu]
> > Sent:         Thursday, April 05, 2001 10:28 AM
> > To:           histonet@pathology.swmed.edu
> > Subject:           storing controls
> >
> > Thanks to all who responded about my IHC decreased staining post.
> >
> > Several people have mentioned storage of the controls.  Some are
> purchased
> > slides, so obviously they are precut and they are stored in the
> > refrigerator.  But most are freshly cut from blocks which are stored at
> > room temperature.  I was under the impression most people store blocks
at
> > room temperature, is this correct?  How long can controls last?  We just
> > started doing histology so our oldest blocks are only 6 months old.
> >
> >
> > Michelle
> >
> >
> > Michelle Peiffer
> > *************************************************************
> > Electron Microscope Facility for the Life Sciences
> > Penn State University Biotechnology Institute
> > 001 South Frear Lab
> > University Park PA 16802
> >
> > phone: 814-865-0212
> > email:  mlk101@psu.edu
> > **************************************************************
> >
> >
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 15:00:40 -0600
> From: Amos & Theresa <atbrooks@snet.net>
> Subject: Re: myogenin
>
> Hi Martha,
>     DAKO has a good antibody (M3559). Our dilution is 1:200. We pretreat
> with a citrate buffer in a steamer for 20 min. Our detection kit is LSAB+,
> but I would assume with a little tweaking of the dilution you could get
> just as good results on the Ventana.
> Amos Brooks
>
> Martha Ward wrote:
>
> > Martha Ward writes:
> >
> > I wonder if someone could share their procedure, vendor, etc. for
> > myogenin?  At one point we had this working but all of a sudden it has
> > quit.  We need a procedure that can be performed on a Ventana ES.
> > Thanks in advance for your help!
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 15:00:59 -0600
> From: KoellingR@immunex.com
> Subject: RE: storing controls
>
> Marjorie,
> This reply is similar to what I sent out to Cheryl George a few minutes
> back although it has not reached the Histonet yet.
>
> What are your thoughts abought storing a positive control block in
> refrigerator for a month (presumably to retain its "antigenicity" and nice
> staining) and then cutting it and using  it as a control for a test or
> patient block that has been sitting at room temp for a month? Unless all
> blocks are stored in refrigerator.
>
> Thanks,
>
> Ray
> Still in Seattle
>
>
>
>
>                     "Hagerty,
>
>                     Marjorie A."         To:     'Michelle Peiffer'
> <mlk101@psu.edu>,
>                     <mhagerty@emc        histonet@pathology.swmed.edu
>
>                     .org>                cc:
>
>                                          Subject:     RE: storing controls
>
>                     04/05/01
>
>                     09:29 AM
>
>
>
>
>
>
>
>
> Michelle,
>
> I am not sure how long controls last, I have not done a scientific study
as
> I am sure some have. Through experience we have found that paraffin
blocks,
> even unsealed, seem to retain antigenicity for a very, very long time.
> Years. As for cut control slides - for some antigens (HER2) cut sections
> can
> remain at room temperature for just a matter of days before the
> antigenicity
> starts to decline. Immuno control slides deteriorate faster if they have
> been incubated or stored at room temperature. From my experience, I would
> recommend that controls slides be cut, but not incubated, and stored in
the
> refrigerator under dry conditions. The expiration on these slides is up
for
> grabs, I would be interested in what others have to say. We go through our
> breast marker controls very rapidly and all others have a 1 year
expiration
> after they are cut. However, we go through the controls in a matter of a
> few
> months.
>
> Good luck,
> Marg
>
> Marjorie Hagerty H.T. (ASCP) H.T.L., Q IHC
> Supervisor, Anatomic Pathology
> Eisenhower Medical Center
> 39-000 Bob Hope Drive
> Rancho Mirage, CA 92270
>
>
> Marg
>
>
> - -----Original Message-----
> From: Michelle Peiffer [mailto:mlk101@psu.edu]
> Sent: Thursday, April 05, 2001 7:29 AM
> To: histonet@pathology.swmed.edu
> Subject: storing controls
>
>
> Thanks to all who responded about my IHC decreased staining post.
>
> Several people have mentioned storage of the controls.  Some are purchased
> slides, so obviously they are precut and they are stored in the
> refrigerator.  But most are freshly cut from blocks which are stored at
> room temperature.  I was under the impression most people store blocks at
> room temperature, is this correct?  How long can controls last?  We just
> started doing histology so our oldest blocks are only 6 months old.
>
>
> Michelle
>
>
> Michelle Peiffer
> *************************************************************
> Electron Microscope Facility for the Life Sciences
> Penn State University Biotechnology Institute
> 001 South Frear Lab
> University Park PA 16802
>
> phone: 814-865-0212
> email:  mlk101@psu.edu
> **************************************************************
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 15:26:47 -0600
> From: RSRICHMOND@aol.com
> Subject: Re: cryosectioning large bone samples
>
> John Baker asks:
>
> >>Does anyone have any tips that might help me cryosection large bone
> samples? The tissue is human navicular and cuneiform bones.<<
>
> hmm, I remember from freshman anatomy 40 years ago -
> Never .... .... .... - .... .... come ....
> (actually I learned it from my father, who took freshman anatomy in 1928.)
>
> Just out of curiosity, where do these specimens come from, and what's
being
> looked for?
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 16:59:53 -0600
> From: Abizar Lakdawalla <abizarl@innogenex.com>
> Subject: Re: Database examples:  Revalidating immuno reagents
>
> Hi Tim, I thought you were a FileMaker fan! 8^}
> Abizar
> www.innogenex.com
>
> "Morken, Tim" wrote:
>
> > I have had a lot of people ask for an example of the antibody info sheet
I
> > mentioned (below). I will send printouts via email to those people, but
I
> > thought anyone interested in that sort of thing would be interested in
the
> > workshop I will be giving at the NSH convention in September. It will
cover
> > developing databases in MS-Access and will specifically cover
applications
> > to IHC and other lab testing and documentation. This will be an advanced
> > class and will assume you have used Access before.
> >
> > Tim
> >
> > -----Original Message-----
> > From: Morken, Tim [mailto:tim9@cdc.gov]
> > Sent: Thursday, April 05, 2001 9:36 AM
> > To: 'Histonet'
> > Subject: RE: Revalidating immuno reagents
> >
> > Joe, CLIA was satisfied with our records showing previous uses of
> > antibodies. I developed a database that keeps track of all experiments
and
> > can collate all uses of any antibody into an "antibody information
sheet."
> > This sheet shows all information about a particular antibody, including
all
> > previous uses of that antibody, slide by slide, and the results of those
> > uses, including comments about performance.
> >
> > Tim Morken, BA, EMT(MSA), HTL(ASCP)
> > Infectious Disease Pathology Activity
> > Centers for Disease Control and Prevention
> > Ms-G32
> > 1600 Clifton Road
> > Atlanta, GA 30333
> > USA
> >
> > PH: 404-639-3964
> > FAX: 404-639-3043
> >
> > email: tim9@cdc.gov
> >
> > -----Original Message-----
> > From: Nocito, Joseph [mailto:joseph_nocito@srhc.iwhs.org]
> > Sent: Thursday, April 05, 2001 8:38 AM
> > To: 'Histonet'
> > Subject: Revalidating immuno reagents
> >
> > Good morning all,
> >         During the last two days, we have been inspected by CLIA. The
> > inspector found an expired immuno reagent in the refrigerator (I know,
shame
> > on me).  I explained to this person that although the reagent is
expired, I
> > always run a known positive control with each run.  If the control
didn't
> > work, the case is repeated with another lot number. This was not good
enough
> > for this person. She wanted proof.  Short of performing a short immuno
run
> > in front of this person, I couldn't site any references.
> >         Does any one have a procedure that can be referenced dealing
with
> > validaing and re-validating reagents?  I'll also mention that each
primary
> > antibody gets titered upon receipt and each new detection kit gets
tested
> > and the results recorded before it is put into use.
> >         Thanks in advance.
> >
> > Joe Nocito, B.S., HT(ASCP)QIHC
> > Histology Supervisor
> > Christus Santa Rosa Hospitals
> > San Antonio, Texas
>
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 17:15:42 -0600
> From: "ANN MARUSKA" <amarusk1@FAIRVIEW.ORG>
> Subject: HSV control
>
> Hi Histonetters,
>
> Anyone out there with an HSV control block that they could spare.......I
will
> trade CMV controls (mine are packed) - my 3 for your 1.  Interested?
>
>
>
> Ann Maruska
> Fairview-University Medical Center
> Mpls. MN  55454
> amarusk1@fairview.org
> 612-273-9119
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> - --=_772C8C1E.2342391E
> Content-Type: text/plain; charset=US-ASCII
> Content-Transfer-Encoding: quoted-printable
> Content-Disposition: inline
>
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --=_772C8C1E.2342391E
> Content-Type: text/plain
> Content-Disposition: attachment; filename="ANN MARUSKA.vcf"
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:MARUSKA, ANN
> EMAIL;WORK;PREF;NGW:AMARUSK1.EAST.NORTH
> X-GWUSERID:AMARUSK1
> ORG:;LAB
> N:MARUSKA;ANN
> TEL;WORK:612-273-9119
> END:VCARD
>
>
> - --=_772C8C1E.2342391E--
>
>
> ----------------------------------------------------------------------
>
> Date: 5 Apr 2001 17:15:59 -0600
> From: DDittus787@aol.com
> Subject: Re: storing controls
>
>
> Dear Michelle
> many antigen sites react adversly to room temp storage, you can get longer
> shelf life, by cutting , bake for only 15 min at 59 degrees, store at 4
> degree refrig in slide boxes.
Dana
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
>
> - --part1_83.931c9fd.27fe4928_boundary
> Content-Type: text/plain; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --part1_83.931c9fd.27fe4928_boundary
> Content-Type: text/html; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
> <HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>Dear Michelle
> <BR>many antigen sites react adversly to room temp storage, you can get
longer
>
> <BR>shelf life, by cutting , bake for only 15 min at 59 degrees, store at
4
> <BR>degree refrig in slide boxes.
>
            &nbs
p;            &n
bsp;            
    &
>
> nbsp;Dana</FONT></HTML>
>
> - --part1_83.931c9fd.27fe4928_boundary--
>
>
> Here are the messages received yesterday!




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