RE: Ventana dispensers leaking

From:"Hagerty, Marjorie A." <mhagerty@emc.org>

Hi Dana,
 
I actually had an S100 dispenser leak so badly during a run that the
negative control was as positive as the positive control. Had to discard the
entire run. I assume the antibody contaminated the negative slide while
travelling? Scary. They did tell me that the dispenser that leaked the worst
was the S100.

Marg 

 

-----Original Message-----
From: DDittus787@aol.com [mailto:DDittus787@aol.com]
Sent: Wednesday, April 11, 2001 3:42 PM
To: Hagerty, Marjorie A.; ckyle@cvdls.ucdavis.edu; Andickert@aol.com;
jifess@pahosp.com; histonet@pathology.swmed.edu; DonnaWillis@texashealth.org
Subject: Re: Ventana dispensers leaking


Marge 
Since the dispensing well sits over the barcode slide only , it is highly 
unlikely that you would get any leaking over to another slide, the problem
is 
in the other direction, did so much leak out that it did not hit slide at 
all, did it only dispense 100ul, or more 
, and can the dispensers (detection as well) run dry and you have no
staining 
at all(like a dry secondary you may have not sight checked? 
                                               Dana 




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