How to section undecalcified frozen bone?

From:Kim Kusser <>


I would like to cut mouse skulls (sans lower jaw, and front incissors) without decalcifing them first. Has anyone out there done something like this, or have an idea of how it could be accomplished?

The purpose of this is to stain NALT (nasal associated lymphoid tissue). This generally involves several lymphoid markers (CD3, Thy1.2, IgD, B220, CD21, CD11C...basically anything we can think of : ). BTW, I also multiple label my stuff. 2 for chromogens, and 3 for fluorescence. Hence, the reason why I prefer frozen. 

Thanks in advance


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