High background

From:Gayle Callis <uvsgc@montana.edu>

Somehow your message is incomplete, see below.

I got lots of background in oral epithelium using ABC method. I stained
frozen section with a rabbit poly-clonal anti-human antibody. The antibody
was not commercial now. I have applied high dilution 1 to 20 goat serum
for1 hour for blocking. The colo 

Any suggestions will be very thankful.


More information on your technic would be nice, buffers, detergents, what
kind of secondary, avidin/biotin blocking, chromogen, diluents for
secondary, etc?????  Species of tissues being stained, I am assuming human???

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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