High background
From: | Gayle Callis <uvsgc@montana.edu> |
Somehow your message is incomplete, see below.
***********************************************
I got lots of background in oral epithelium using ABC method. I stained
frozen section with a rabbit poly-clonal anti-human antibody. The antibody
was not commercial now. I have applied high dilution 1 to 20 goat serum
for1 hour for blocking. The colo
Any suggestions will be very thankful.
************************************************
More information on your technic would be nice, buffers, detergents, what
kind of secondary, avidin/biotin blocking, chromogen, diluents for
secondary, etc????? Species of tissues being stained, I am assuming human???
Thanks
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
406 994-6367
404 994-4303 (FAX)
<< Previous Message | Next Message >>