Help How to get rid of background

From:jian ma <>

<html><DIV> <P>Hi, all:</P> <P>I got lots of background in oral epithelium using ABC method. I stained frozen section with a rabbit poly-clonal anti-human antibody. The antibody was not commercial now. I have applied high dilution 1 to 20 goat serum for1 hour for blocking. The color was developed in DAB for 30 second to 1 minute, The background staining in the epithelium still can be seen. It was said that rabbit serum, but not mice and goat, contains environmental antibodies to human epithelium or Fossman antigen reaction. Is it impossible to get rid of background?</P> <P>Any suggestions will be very thankful.</P> <P>Jian Ma</P></DIV><br clear=all><hr>Get Your Private, Free E-mail from MSN Hotmail at <a href=""></a>.<br></p></html>
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