-----Original Message-----
From: Ruegg Patsy 
Sent: Monday, April 02, 2001 10:48 AM
To: 'Patty Lott'
Subject: Solochrome Azurine for AL


I purchased this stain from a German Company i believe, I think it was
distributed by ROBOZ
Pfaltz & Bauer, Inc.
Solochrome Azurine, C.I. No. 43830
Cat. No. S07772
 
good luck, it is not easy to find
 
Patsy Ruegg

-----Original Message-----
From: Patty Lott [mailto:Patty.Lott@ORTHO.UAB.EDU]
Sent: Monday, April 02, 2001 9:29 AM
To: 'Patsy.Ruegg@UCHSC.edu'
Subject: RE: stain for aluminum in plastic sections



Thanks so much! 

Say, where did you buy the solochrome azurine?  (I don't see it in Aldrich,
which is my usual.) 

-----Original Message----- 
From: Patsy.Ruegg@UCHSC.edu [ mailto:Patsy.Ruegg@UCHSC.edu
<mailto:Patsy.Ruegg@UCHSC.edu> ] 
Sent: Friday, March 30, 2001 7:48 PM 
To: Patty.Lott@ORTHO.UAB.EDU; Patsy.Ruegg@UCHSC.edu 
Cc: histonet@pathology.swmed.edu 
Subject: RE: stain for aluminum in plastic sections 


Patty, 
my AL procedure is very simple 
make up a 0.5% solution of solochrome azurine in dih20 
ph to 5 with weak acetic acid i believe (remind me to check this) 
the solution will turn red (filter solution) 
stain the gma sections in this solution for 1 to 12 hours at RT 
pour off the stain and rinse very well in dih20 
pour on a solution of 0.4M EDTA ph 7.4 for 30 min. 
rinse well with dih20 
pour on another change of 0.4M EDTA ph 7.4 for 30 min. 
rinse well in dih20 and then let sit in dih20 for 5 min. until clear 
rinse again 
ary dry 
coverslipp with permount 
results: 
AL will appear as blue deposit lines around bone 
it is much like tetracycline in that it goes to where new bone is being 
formed and deposits there, it is much more detramental than tet. though as 
it prevents the formation of anymore bone after it is deposited, so it must 
be chelated or the bones go to hell 

Iron can do the same thing and deposit at the same mineralization front as 
AL, so you must do an iron stain at the same time to confirm that the AL 
positive reaction is really AL and not iron.  The iron stain does not stain 
AL.  We published a paper on this a few years ago in JOH, I will find the 
reference for you.  

-----Original Message----- 
From: Patty Lott 
To: 'Patsy.Ruegg@uchsc.edu' 
Sent: 3/30/01 9:51 AM 
Subject: stain for aluminum in plastic sections 

Could I please get a copy of your procedure?  I'd love to try it!  (I've 
already ordered the reagents for the other, but if this is much 
better--which is what I am hearing--I need to know it!) 

Thanks! 

Patty Lott 
Lab.Supervisor 
Orthopaedic Research Laboratory 
University of Alabama at Birmingham