glycol methacrylate sections
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From: | MarikoKoe@aol.com |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
Would anyone with some fairly extensive GMA (or other plastics) viewing
experience care to briefly discuss the following? What is your comfort level
with being assured of the thickness of the section? That is, if you are
looking at a well-processed, well sectioned, well-stained kidney biopsy, how
confident are you to state that this section is 1.5 microns versus 2.0
microns thick by microscopic viewing alone. Note I am not talking about
cutting one at 1.5 and another at 2.0 and then guessing which might look a
little thinner. But what about viewing a single slide and stating with
confidence that this section is at 1.0 or 1.5 or 2.0 microns? Where is your
comfort level for making this assessment? In addition, what is your idea of
slide readability for diagnostic purposes? If something is requested at 1.0
to 2.0 microns, is 2.5 microns "too thick" to read? Obviously this may have
to do with the specimen and the pathology involved, but how precisely crucial
do you consider the thickness of the section to within a micron or so of the
requested value?
Thanks,
Ray in Seattle
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