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From:meadows <>
To:HistoNet Server <>
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Hello everyone!

I'm hoping that someone out there has had some experience with staining for 
beta-gal on paraffin embedded tissue sections.  We've gotten it to work on 
frozens but I've been spoiled with paraffins and would prefer to use them 
instead. The only problem is that the beta-gal doesn't seem to be as eager as 
I am to switch over.  Any advice (recommended antibody to use, detection 
method, pretreatment of tissue, etc.) would be greatly appreciated.  Thank you 
for all your help.


cell biology and anatomy
medical college of georgia
augusta, Georgia

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