Ventana ES & genII users

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From:"Drew Sally A." <>
To:'Histonet' <>

We were wondering what type of counterstain do you use on your
instrument...Do you use Ventana's hematoxylin or a homebrew?  We have been
using a homebrew-diluted commercial Harris hematoxylin.  Our diluent is
composed of 128 mL Type 1 water, 60 mL 20% polyethylene glycol, and 
12 mL glacial acetic acid.  Since we feel the diluted stain is fading too
much in the dispensers, we're kind of looking for a replacement protocol.
We would welcome any and all suggestions!
Thank you!

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI 

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