I just recently discovered this list server, and am interested in a topic 
that I believe was discussed previously. Rather than start a repetitive 
dialogue, I would appreciate if someone could let me know the approximate 
dates during which troubleshooting with antibody thawing was discussed. Or if 
I am mistaken, and this hasn't been discussed recently.... I am having a 
problem with some antibodies used in staining of brain tissue -some of them 
just aren't lasting as long as would be expected. I haven't been able to pin 
point why this is occurring, but I heard there was talk that there is a text 
book way of thawing the antibodies for making dilutions. Any help in this 
area would be greatly appreciated. 
~Anju
Washington University Medical School
St. Louis, MO