Staining Procedure used the BSC

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From:bogerink@wfubmc.edu (Hermina Bogerink)
To:"rkline@emscience.com" <rkline@emscience.com>, histonet <histonet@pathology.swmed.edu>
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Rande,

I do not have a copy of the 2nd edition of Staining Procedures.
However, I do have a hand-typed copy of a Gram-Weigert positive stain
that probably is older than I would like to admit!!  Both gentian violet
and crystal violet are primarily used in gram-positive stains.  My
procedure utilizes crystal violet and is a modification of the
Brown-Brenn method.  If you would like me to fax you a copy, please let
me know.  As for Verhoeff's Carbol Fuchsin,  it is in Selected
Histochemical and Histopathological Methods, p. 1032 (1966).  I can make
you a photocopy of that as well if you do not have access to this book.
This is a lengthy protocol and I just do not have the time to include it
with this e-mail.

Hermina Borgerink, BA, HT(ASCP)IHQ
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27127
ph (336) 716-1538
fax (336) 716-1515







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