Re: ihc for amyloid

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From:Shelley Sheridan <>
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<P>Patsy and Phyllis, </P> <P>We do all of our IHC by hand so I don't have any information about an autostainer.  We use an ABC procedure with DAB.  All of our formalin fixed tissue gets pretreated for 1 minute in 88% Formic Acid for antigen retreival.  We use a wide assortment of antibodies, most of which we make here, but there are a few that we use that are commercially available.  6E10, 4G8 are two beta-Amyloid antibodies that come to my mind.  If you want more information about these antibodies just let me know.</P> <P> </P><BR><BR>Shelley K. Sheridan<br>Research Specialist<br>Center for Neurodegenerative Disease Research<br>University of Pennsylvania Medical School<br>Philadelphia, PA 19104<br>Phone:(215)614-0051<p><br><hr size=1><b>Do You Yahoo!?</b><br> Send online invitations with <a href="">Yahoo! Invites</a>.
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