Joyce Kotzuk wrote:

> GFAP works well in frozen sections, and shows astrocytes and their processes (fibers). I think I used a fairly low concentration, like 1:5000, but you would probably want to do a dilution curve for your tissue anyway.
> Joyce Kotzuk, UNM pathology

Very true, but not all astrocytes are GFAP positive.

>
> >>> Atoska Gentry <gentras@vetmed.auburn.edu> 04/13/00 09:16AM >>>
> >Date: Wed, 12 Apr 2000 16:33:11 -0500 (CDT)
> >>
> >
> >Help Pam Marcum, Gayle Callis and/or any of you other frozen brain sections
> >experts.  Now that we are finally getting decent readable sections another
> >problem surfaces.  After completing my H&E's the nuclei are very visible
> >but can any of you recommend a stain/stains that would reveal cell margins
> >(i.e. whereby astrocytes, and glial fibers are distinguishable)?  thanks,
> >Atoska
> >
> >Atoska S. Gentry B.S., HT(ASCP)
> >Research Assistant
> >Scott-Rtichey Research Center
> >College of Veterinary Medicine
> >Auburn University, AL  36849
> >PH (334) 844-5579
> >FAX (334) 844-5850
> >

Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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