Hi Barry,
indeed this formalin-fixation story has been discussed numerous times in our
lab. IŽd be glad if you can give me some details on this phenomenon.
Thanks !

Jochen Schuck

> Von: Barry Rittman <brittman@mail.db.uth.tmc.edu>
> Antworten an: histonet@pathology.swmed.edu
> Datum: Wed, 19 Apr 2000 11:11:51 -0500
> An: histology <histonet@pathology.swmed.edu>
> Betreff: Re: an interesting question has come up
> 
> Gayle,
> this situation arises because initially formalin kills the cells, produces
> "temporary" bonds between various groups and then forms more permanent
> "irreversible" bonds. the irreversible bond formation continues as long as
> tissue is in formalin.
> After the formation of the "temporary" or weak bonds, the process can be
> reversed by procedures such as washing in water as this prevents the formation
> of the irreversible bonds. Can give you more details if you wish.
> Barry
> 
> Gayle Callis wrote:
> 
>> Have been a private discussion with someone, who brought up the point that
>> fixation can reverse itself, and in the context of formalin?  but that the
>> fixation process is eventually irreversible.
>> 
>> Any comments on this, I found it interesting, and know the other party is
>> looking in.
>> 
>> I have never experienced this, or even heard of it happening, but am always
>> ready for enlightenment, could be something I have missed in reading,
>> listening or discussion.
>> 
>> Gayle Callis
>> Veterinary Molecular Biology
>> Montana State University
>> Bozeman MT 59717-3610
>> 406 994-4705
>> 406 994-4303
> 
>