Re: Thawing of Antibodies

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From:"Tim Morken" <>
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Aliquot the antibody and then freeze at -70. After that you need only take 
out what you need for an experiment.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


Phone: (404) 639-3964
FAX:  (404)639-3043

----Original Message Follows----
Subject: Thawing of Antibodies
Date: Thu, 27 Apr 2000 09:19:26 EDT

I just recently discovered this list server, and am interested in a topic
that I believe was discussed previously. Rather than start a repetitive
dialogue, I would appreciate if someone could let me know the approximate
dates during which troubleshooting with antibody thawing was discussed. Or 
I am mistaken, and this hasn't been discussed recently.... I am having a
problem with some antibodies used in staining of brain tissue -some of them
just aren't lasting as long as would be expected. I haven't been able to pin
point why this is occurring, but I heard there was talk that there is a text
book way of thawing the antibodies for making dilutions. Any help in this
area would be greatly appreciated.
Washington University Medical School
St. Louis, MO

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