aNJU,

Aliquot the antibody and then freeze at -70. After that you need only take 
out what you need for an experiment.


Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043



----Original Message Follows----
From: Unju@aol.com
To: histonet@pathology.swmed.edu
Subject: Thawing of Antibodies
Date: Thu, 27 Apr 2000 09:19:26 EDT

I just recently discovered this list server, and am interested in a topic
that I believe was discussed previously. Rather than start a repetitive
dialogue, I would appreciate if someone could let me know the approximate
dates during which troubleshooting with antibody thawing was discussed. Or 
if
I am mistaken, and this hasn't been discussed recently.... I am having a
problem with some antibodies used in staining of brain tissue -some of them
just aren't lasting as long as would be expected. I haven't been able to pin
point why this is occurring, but I heard there was talk that there is a text
book way of thawing the antibodies for making dilutions. Any help in this
area would be greatly appreciated.
~Anju
Washington University Medical School
St. Louis, MO





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