Re: Tellyesniczky's fixative
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Thu, 27 Apr 2000 RSRICHMOND@aol.com wrote:
> Tellyesniczky's fixative (boy, do I love copy-and-paste) appears to be
>one of
> many fixatives, published and proprietary, of approximately this
>composition.
> As I've posted before, I use Davidson's fixative (3 parts water, 3 parts
> waste ethanol, 2 parts 37% formaldehyde, 1 part glacial acetic acid) for
>such
> purposes.
But Bob, are they really equivalent? The major differences among these
AFA mixtures are in the amounts of water and alcohol. There's always
enough acetic acid to give a clean chromatin appearance, and enough
formaldehyde to immobilize the proteins (if it's given enough time).
Most AFA fixatives contain somewhere around 75-85% alcohol and about
20% water, by volume. Davidson's is more than half water, and only
about 30-35% alcohol. This alcohol concentration is not high enough to
bring about rapid coagulant fixation of all proteins. Gin, whisky etc
all contain 40%, and they don't kill off the gustatory receptors.
A professional taster, with his notebook and spitoon, exposes his
tongue to something comparable to Davidson's (without formalin and
acetic acid) for several hours every day.
John Kiernan
London, Canada.
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