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From:TGAco@aol.com
To:brett_connolly@merck.com, histonet@pathology.swmed.edu
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Brett-
We typically use BSA blocker when performing murine based ELISA's.  Also, 
titering the secondary antibody until you find a level at which the 
background is low, but the controls and/or standard curves remain linear.  We 
run these types of assays in high throughput situations and have a handle on 
this background problem.  Feel free to contact me at your convenience should 
you have any other questions.  Good luck.

Michael Settles
TGA Sciences, Inc.
781-393-6910
www.tgasciences.com



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