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From: | TGAco@aol.com |
To: | brett_connolly@merck.com, histonet@pathology.swmed.edu |
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Content-Type: | text/plain; charset="US-ASCII" |
Brett-
We typically use BSA blocker when performing murine based ELISA's. Also,
titering the secondary antibody until you find a level at which the
background is low, but the controls and/or standard curves remain linear. We
run these types of assays in high throughput situations and have a handle on
this background problem. Feel free to contact me at your convenience should
you have any other questions. Good luck.
Michael Settles
TGA Sciences, Inc.
781-393-6910
www.tgasciences.com
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