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We typically use BSA blocker when performing murine based ELISA's.  Also, 
titering the secondary antibody until you find a level at which the 
background is low, but the controls and/or standard curves remain linear.  We 
run these types of assays in high throughput situations and have a handle on 
this background problem.  Feel free to contact me at your convenience should 
you have any other questions.  Good luck.

Michael Settles
TGA Sciences, Inc.

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